ArticlePLoS neglected tropical diseases2014
Aquaporins are critical for provision of water during lactation and intrauterine progeny hydration to maintain tsetse fly reproductive success.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Viviparity and obligate blood feeding: tsetse flies as a unique research system to study climate change.Current opinion in insect science · 2025Review
- Pathogen-Mediated Alterations of Insect Chemical Communication: From Pheromones to Behavior.Pathogens (Basel, Switzerland) · 2023Review
- Dehydration Dynamics in Terrestrial Arthropods: From Water Sensing to Trophic Interactions.Annual review of entomology · 2023Review
- Article
- Potential Galactagogue Effect of Tri-Than-Thip Remedy on Milk Synthesis in Lactating Rats: Impact on Milk Production-Related Parameters.International journal of medical sciences · 2023Article
- Warm Blood Meal Increases Digestion Rate and Milk Protein Production to Maximize Reproductive Output for the Tsetse Fly,Insects · 2022Article
- Reactive Oxygen Species Mediate Activity-Regulated Dendritic Plasticity Through NADPH Oxidase and Aquaporin Regulation.Frontiers in cellular neuroscience · 2021Article
- Polyherbal formula (ASILACT®) induces Milk production in lactating rats through Upregulation of α-Lactalbumin and aquaporin expression.BMC complementary medicine and therapies · 2020Article
- Enhancing RNAi Efficiency to Decipher the Functional Response of Potential Genes inFrontiers in physiology · 2020Article
- Article
- Survey for positively selected coding regions in the genome of the hematophagous tsetse fly Glossina morsitans identifies candidate genes associated with feeding habits and embryonic development.Genetics and molecular biology · 2020Article
- Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).Scientific reports · 2019Article
- A Novel Aquaporin 12-like Protein fromGenes · 2019Article
- An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua.Scientific reports · 2019Article
- Identification, Expression Patterns and RNA Interference of Aquaporins inFrontiers in physiology · 2019Article
- Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives.BMC microbiology · 2018Article
- The genome of the water strider Gerris buenoi reveals expansions of gene repertoires associated with adaptations to life on the water.BMC genomics · 2018Article
- Muscles provide an internal water reserve for reproduction.Proceedings. Biological sciences · 2018Article
- Dehydration prompts increased activity and blood feeding by mosquitoes.Scientific reports · 2018Article
- Identification and Functional Analysis of the First Aquaporin from Striped Stem Borer,Frontiers in physiology · 2018Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Tsetse flies undergo drastic fluctuations in their water content throughout their adult life history due to events such as blood feeding, dehydration and lactation, an essential feature of the viviparous reproductive biology of tsetse. Aquaporins (AQPs) are transmembrane proteins that allow water and other solutes to permeate through cellular membranes. Here we identify tsetse aquaporin (AQP) genes, examine their expression patterns under different physiological conditions (blood feeding, lactation and stress response) and perform functional analysis of three specific genes utilizing RNA interference (RNAi) gene silencing. Ten putative aquaporins were identified in the Glossina morsitans morsitans (Gmm) genome, two more than has been previously documented in any other insect. All organs, tissues, and body parts examined had distinct AQP expression patterns. Two AQP genes, gmmdripa and gmmdripb ( = gmmaqp1a and gmmaqp1b) are highly expressed in the milk gland/fat body tissues. The whole-body transcript levels of these two genes vary over the course of pregnancy. A set of three AQPs (gmmaqp5, gmmaqp2a, and gmmaqp4b) are expressed highly in the Malpighian tubules. Knockdown of gmmdripa and gmmdripb reduced the efficiency of water loss following a blood meal, increased dehydration tolerance and reduced heat tolerance of adult females. Knockdown of gmmdripa extended pregnancy length, and gmmdripb knockdown resulted in extended pregnancy duration and reduced progeny production. We found that knockdown of AQPs increased tsetse milk osmolality and reduced the water content in developing larva. Combined knockdown of gmmdripa, gmmdripb and gmmaqp5 extended pregnancy by 4-6 d, reduced pupal production by nearly 50%, increased milk osmolality by 20-25% and led to dehydration of feeding larvae. Based on these results, we conclude that gmmDripA and gmmDripB are critical for diuresis, stress tolerance and intrauterine lactation through the regulation of water and/or other uncharged solutes.
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