ArticleNature protocols2014
In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- Neural Expression of IL-16 Mediates Neuropathic Pain after Sciatic Injury in Mice.Molecular neurobiology · 2026Article
- Initial efforts of translational development of AAV-encoded NaMolecular therapy. Advances · 2026Article
- Selective RNAi silencing of Schwann cell Piezo1 alleviates mechanical hypersensitization following peripheral nerve injury.Molecular therapy. Methods & clinical development · 2025Article
- PAK2 is necessary for myelination in the peripheral nervous system.Brain : a journal of neurology · 2024Article
- Selective RNAi-silencing of Schwann cell Piezo1 alleviates mechanical hypersensitization following peripheral nerve injury.Research square · 2023Article
- Toward peripheral nerve mechanical characterization using Brillouin imaging spectroscopy.Neurophotonics · 2023Article
- The transcription factor Stat-1 is essential for Schwann cell differentiation, myelination and myelin sheath regeneration.Molecular medicine (Cambridge, Mass.) · 2023Article
- Models and methods to study Schwann cells.Journal of anatomy · 2022Review
- Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses.Journal of neuroinflammation · 2022Article
- Traumatic and Diabetic Schwann Cell Demyelination Is Triggered by a Transient Mitochondrial Calcium Release through Voltage Dependent Anion Channel 1.Biomedicines · 2022Article
- Physiology of PNS axons relies on glycolytic metabolism in myelinating Schwann cells.PloS one · 2022Article
- Metabolic Control of Sensory Neuron Survival by the p75 Neurotrophin Receptor in Schwann Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Reprogramming an energetic AKT-PAK5 axis boosts axon energy supply and facilitates neuron survival and regeneration after injury and ischemia.Current biology : CB · 2021Article
- AAV2/9-mediated silencing of PMP22 prevents the development of pathological features in a rat model of Charcot-Marie-Tooth disease 1 A.Nature communications · 2021Article
- Targeted PMP22 TATA-box editing by CRISPR/Cas9 reduces demyelinating neuropathy of Charcot-Marie-Tooth disease type 1A in mice.Nucleic acids research · 2020Article
- Exosomes derived from high-glucose-stimulated Schwann cells promote development of diabetic peripheral neuropathy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Lentivirus-Mediated RNA Interference Targeting RhoA Slacks the Migration, Proliferation, and Myelin Formation of Schwann Cells.Molecular neurobiology · 2017Article
- In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation.Journal of visualized experiments : JoVE · 2016Article
- Sulforhodamine 101, a widely used astrocyte marker, can induce cortical seizure-like activity at concentrations commonly used.Scientific reports · 2016Article
- Optimal myelin elongation relies on YAP activation by axonal growth and inhibition by Crb3/Hippo pathway.Nature communications · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The myelin sheath is essential for the rapid and efficient propagation of action potentials. However, our understanding of the basic molecular mechanisms that regulate myelination, demyelination and remyelination is limited. Schwann cells produce myelin in the peripheral nervous system and remain associated with the axons of peripheral neurons throughout axonal migration to the target. Owing to the intimate relationship between these cell types it is difficult to fully reproduce their function in vitro. For this reason, we developed an approach based on the injection of an engineered virus into the sciatic nerve of mice to locally transduce peripheral nerve cells. This approach can be used as an alternative to germline transgenesis to facilitate the investigation of peripheral nerve biology in vivo. The detailed protocol, described here, requires 3 weeks to complete. In comparison with genetic modification strategies, this protocol is a fast, reproducible and straightforward method for introducing exogenous factors into myelinating Schwann cells and myelinated axons in vivo to investigate specific molecular mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.