ArticleAnalytical chemistry2014
Microfluidic bead suspension hopper.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Continuous Size-Based Particle Separation Using Inertial Force and Deterministic Lateral Displacement.Micromachines · 2026Article
- A sticky situation - simple method for rapid poissonian encapsulation of highly aggregation-prone microbeads in polydisperse emulsions.Frontiers in bioengineering and biotechnology · 2025Article
- Offsetting Dense Particle Sedimentation in Microfluidic Systems.Micromachines · 2024Article
- Hydrogel-Encapsulated Beads Enable Proximity-Driven Encoded Library Synthesis and Screening.ACS central science · 2023Article
- Shaking Device for Homogeneous Dispersion of Magnetic Beads in Droplet Microfluidics.Sensors (Basel, Switzerland) · 2023Article
- Translating the Genome into Drugs.Accounts of chemical research · 2023Article
- Picoliter Droplet Generation and Dense Bead-in-Droplet Encapsulation via Microfluidic Devices Fabricated via 3D Printed Molds.Micromachines · 2022Article
- Considerations for Achieving Maximized DNA Recovery in Solid-Phase DNA-Encoded Library Synthesis.ACS combinatorial science · 2020Article
- Droplet CAR-Wash: continuous picoliter-scale immunocapture and washing.Lab on a chip · 2019Article
- Poisson Statistics of Combinatorial Library Sampling Predict False Discovery Rates of Screening.ACS combinatorial science · 2017Article
- An Integrated Microfluidic Processor for DNA-Encoded Combinatorial Library Functional Screening.ACS combinatorial science · 2017Article
- A Label-free Sirtuin 1 Assay based on Droplet-Electrospray Ionization Mass Spectrometry.Analytical methods : advancing methods and applications · 2016Article
- hνSABR: Photochemical Dose-Response Bead Screening in Droplets.Analytical chemistry · 2016Article
- Discovery in Droplets.Analytical chemistry · 2016Review
- DNA-Encoded Solid-Phase Synthesis: Encoding Language Design and Complex Oligomer Library Synthesis.ACS combinatorial science · 2015Article
- Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors.Analytical chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Many high-throughput analytical platforms, from next-generation DNA sequencing to drug discovery, rely on beads as carriers of molecular diversity. Microfluidic systems are ideally suited to handle and analyze such bead libraries with high precision and at minute volume scales; however, the challenge of introducing bead suspensions into devices before they sediment usually confounds microfluidic handling and analysis. We developed a bead suspension hopper that exploits sedimentation to load beads into a microfluidic droplet generator. A suspension hopper continuously delivered synthesis resin beads (17 μm diameter, 112,000 over 2.67 h) functionalized with a photolabile linker and pepstatin A into picoliter-scale droplets of an HIV-1 protease activity assay to model ultraminiaturized compound screening. Likewise, trypsinogen template DNA-coated magnetic beads (2.8 μm diameter, 176,000 over 5.5 h) were loaded into droplets of an in vitro transcription/translation system to model a protein evolution experiment. The suspension hopper should effectively remove any barriers to using suspensions as sample inputs, paving the way for microfluidic automation to replace robotic library distribution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.