ArticleProceedings of the National Academy of Sciences of the United States of America2014
Structure of a mitochondrial cytochrome c conformer competent for peroxidase activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 112 citations in OpenAlex.
- A Divergent Mitochondrial Cytochrome c in Malaria Parasites With an Anomalously Low Redox Potential.Journal of molecular biology · 2026Article
- Dimerization-induced conformational transitions of yeast iso-1 cytochromeMagnetic resonance letters · 2026Article
- Yeast mitochondrial adaptation to freeze thaw stress depends on pre freeze fermentation state.NPJ science of food · 2026Article
- Differences in functional cross-talk between loops C and D in two mitochondrial cytochromes.Journal of inorganic biochemistry · 2026Article
- A Divergent CytochromebioRxiv : the preprint server for biology · 2025Article
- Chemical propulsion of hemozoin crystal motion in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Disabling the Entatic Control of Methionine Ligation through Additive Destabilization of Ferric CytochromeInorganic chemistry · 2025Article
- Order-to-Disorder and Disorder-to-Order Transitions of Proteins upon Binding to Phospholipid Membranes: Common Ground and Dissimilarities.Biomolecules · 2025Review
- Evolution of Theories on Doxorubicin-Induced Late Cardiotoxicity-Role of Topoisomerase.International journal of molecular sciences · 2024Review
- The Increase in the Peroxidase Activity of the CytochromeInternational journal of molecular sciences · 2024Article
- Direct tests of cytochromeProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Resonance Raman Studies on Heme Ligand Stretching Modes in Methionine80-Depleted CytochromeThe journal of physical chemistry. B · 2023Article
- Direct Tests of Cytochrome Function in the Electron Transport Chain of Malaria Parasites.bioRxiv : the preprint server for biology · 2023Article
- Alkaline State of the Domain-Swapped Dimer of Human CytochromeJournal of the American Chemical Society · 2022Article
- PB1F2 from Influenza A Virus Regulates the Interaction between Cytochrome C and Cardiolipin.Membranes · 2022Article
- Effect on intrinsic peroxidase activity of substituting coevolved residues from Ω-loop C of human cytochrome c into yeast iso-1-cytochrome c.Journal of inorganic biochemistry · 2022Article
- Cofactor Dynamics Couples the Protein Surface to the Heme in CytochromeThe journal of physical chemistry. B · 2022Article
- Naturally Occurring I81N Mutation in Human CytochromeACS omega · 2022Article
- Interactions between mitochondria-damaging platinum(IV) prodrugs and cytochrome c.Dalton transactions (Cambridge, England : 2003) · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
At the onset of apoptosis, the peroxidation of cardiolipin at the inner mitochondrial membrane by cytochrome c requires an open coordination site on the heme. We report a 1.45-Å resolution structure of yeast iso-1-cytochrome c with the Met80 heme ligand swung out of the heme crevice and replaced by a water molecule. This conformational change requires modest adjustments to the main chain of the heme crevice loop and is facilitated by a trimethyllysine 72-to-alanine mutation. This mutation also enhances the peroxidase activity of iso-1-cytochrome c. The structure shows a buried water channel capable of facilitating peroxide access to the active site and of moving protons produced during peroxidase activity to the protein surface. Alternate positions of the side chain of Arg38 appear to mediate opening and closing of the buried water channel. In addition, two buried water molecules can adopt alternate positions that change the network of hydrogen bonds in the buried water channel. Taken together, these observations suggest that low and high proton conductivity states may mediate peroxidase function. Comparison of yeast and mammalian cytochrome c sequences, in the context of the steric factors that permit opening of the heme crevice, suggests that higher organisms have evolved to inhibit peroxidase activity, providing a more stringent barrier to the onset of apoptosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.