Evidence map›Paper›PMID 24760697›Full record

ArticleJournal of medical virology2014

A novel molecular test for influenza B virus detection and lineage differentiation.

Chloe K S Wong, Gary C H Tsang, Kwok-Hung Chan, Olive T W Li, Malik Peiris, Leo L M Poon

Open access · greenAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Chloe K S WongCentre of Influenza Research and School of Public Health, The University of Hong Kong, Hong Kong, SAR, China.
Gary C H Tsang
Kwok-Hung Chan
Olive T W Li
Malik Peiris
Leo L M Poon
University of Hong Kong · HK

Funding

NIAID NIH HHS HHSN272201400006C
6 · The paper itself

Abstract

Contemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance.

Indexed as

BenzothiazolesDiaminesGenotyping TechniquesHumansInfluenza B virusInfluenza, HumanMolecular Diagnostic TechniquesOrganic ChemicalsPolymerase Chain ReactionQuinolinesRetrospective StudiesSensitivity and SpecificityStaining and LabelingBenzothiazolesDiaminesOrganic ChemicalsQuinolinesSYBR Green Iinfluenza B viruslineage differentiationRT-PCRVictoriaYamagata

Identifiers

PMID24760697
PMCPMC4206597
OpenAlexW1506593894

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.