ArticleMedical molecular morphology2015
The elongation of primary cilia via the acetylation of α-tubulin by the treatment with lithium chloride in human fibroblast KD cells.
Article in Medical molecular morphology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- IFT139 regulates Hedgehog signaling and cilia structure through ciliary protein localization.Biology open · 2025Article
- Impaired Formation of Primary Cilia in Olfactory Neuronal Precursors Is Associated with Decreased Proliferation and Maturation in Individuals with Hyposmia.International journal of molecular sciences · 2025Article
- GDF15 controls primary cilia morphology and function thereby affecting progenitor proliferation.Life science alliance · 2024Article
- Primary cilia suppress the fibrotic activity of atrial fibroblasts from patients with atrial fibrillation in vitro.Scientific reports · 2024Article
- High water intake induces primary cilium elongation in renal tubular cells.Kidney research and clinical practice · 2024Article
- HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2023Article
- Review
- Oligodendroglial primary cilium heterogeneity during development and demyelination/remyelination.Frontiers in cellular neuroscience · 2022Article
- Characterization of Primary Cilia in Osteoblasts Isolated From Patients With ADPKD and CKD.JBMR plus · 2021Article
- Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated capillary.Cell and tissue research · 2021Article
- Loss of Deacetylation Enzymes Hdac6 and Sirt2 Promotes Acetylation of Cytoplasmic Tubulin, but Suppresses Axonemal Acetylation in Zebrafish Cilia.Frontiers in cell and developmental biology · 2021Article
- Alpha-Tubulin Acetylation inFrontiers in cellular and infection microbiology · 2021Article
- Role of tubulin acetylation in cellular functions and diseases.Medical molecular morphology · 2020Review
- Rho-kinase and PKCα Inhibition Induces Primary Cilia Elongation and Alters the Behavior of Undifferentiated and Differentiated Temperature-sensitive Mouse Cochlear Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Ciliotherapy Treatments to Enhance Biochemically- and Biophysically-Induced Mesenchymal Stem Cell Osteogenesis: A Comparison Study.Cellular and molecular bioengineering · 2019Article
- Olfactory Loss and Dysfunction in Ciliopathies: Molecular Mechanisms and Potential Therapies.Current medicinal chemistry · 2019Review
- Dynamic localization of α-tubulin acetyltransferase ATAT1 through the cell cycle in human fibroblastic KD cells.Medical molecular morphology · 2018Article
- Ripor2 is involved in auditory hair cell stereociliary bundle structure and orientation.Journal of molecular medicine (Berlin, Germany) · 2018Article
- Cilium Length and Intraflagellar Transport Regulation by Kinases PKG-1 and GCK-2 in Caenorhabditis elegans Sensory Neurons.Molecular and cellular biology · 2018Article
- Outer dense fibers stabilize the axoneme to maintain sperm motility.Journal of cellular and molecular medicine · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary cilium, an organelle found on nearly every cell in the human body, typically serves as the mechanical sensor of the cell. Lithium ion is known to promote the elongation of primary cilia in a variety of cell types, but it is unknown whether lithium is involved in the acetylation of α-tubulin which is essential for the assembly of primary cilia. In order to reveal the relationship between the elongation of primary cilia with lithium and the acetylation of α-tubulin, we first observed the formation and structure of primary cilia in KD cells, a cell line deriving fibroblasts in human labium. Subsequently, by immunohistochemical and western blot analysis we elucidated that the length of primary cilia and acetylation of α-tubulin are regulated by lithium chloride (LiCl) in the medium in a time- and concentration-dependent manner. We next performed the RT-PCR, RNAi-based experiments and biochemical study using an inhibitor of glycogen synthase kinase-3βGSK-3β). We found that LiCl mobilizes the α-tubulin N-acetyltransferase 1 (αTAT1) in the signaling pathway mediating GSK-3β and adenylate cyclase III. In conclusion, our results suggested that LiCl treatments activate αTAT1 by the inhibition of GSK-3β and promote the α-tubulin acetylation, and then elongate the primary cilia.
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