ArticleInternational urogynecology journal2014
Collagen type III alpha 1 polymorphism (rs1800255, COL3A1 2209 G>A) assessed with high-resolution melting analysis is not associated with pelvic organ prolapse in the Dutch population.
Article in International urogynecology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 3 of them syntheses that pooled it.
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Who cites it
10 citing papers in PubMed, 3 syntheses or guidelines pooled it, 18 citations in OpenAlex.
- Systematic review and meta-analysis of genetic association studies of pelvic organ prolapse.International urogynecology journal · 2022Pooled it
- Pooled it
- Systematic review and metaanalysis of genetic association studies of urinary symptoms and prolapse in women.American journal of obstetrics and gynecology · 2015Pooled it
- COL3A1 Gene Polymorphism and Its Impact on Female Pelvic Organ Prolapse.Medical science monitor : international medical journal of experimental and clinical research · 2025Review
- Genetics of Female Pelvic Organ Prolapse: Up to Date.Biomolecules · 2024Review
- Women with pelvic organ prolapse and fibulin-5 rs12589592 polymorphism.Revista da Associacao Medica Brasileira (1992) · 2024Article
- Advances in molecular mechanisms of pelvic organ prolapse (Review).Experimental and therapeutic medicine · 2021Review
- Common variants in LAMC1 confer risk for pelvic organ prolapse in Chinese population.Hereditas · 2020Article
- Genetic polymorphisms in collagen-related genes are associated with pelvic organ prolapse.Menopause (New York, N.Y.) · 2020Article
- Polymorphism rs1800255 from COL3A1 gene and the risk for pelvic organ prolapse.International urogynecology journal · 2020Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introduction and hypothesisThe rs1800255, COL3A1 2209 G>A polymorphism in the alpha 1 chain of collagen type III has been associated with an increased risk of pelvic organ prolapse (POP). In one of our previous studies however, polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) misdiagnosed rs1800255, COL3A1 2209 G>A in 6 % of cases. The high-resolution melting (HRM) analysis on the contrary obtained a 100 % accordance for this specific polymorphism and was used in the present study to validate this risk factor for POP.
methodsIn this case-control study, women with and without symptoms of POP were included and compared. DNA was extracted from blood samples. HRM analysis was used to assess for the presence of the homozygous rs1800255. Groups were compared using the Pearson chi-square, Mann-Whitney, and t tests. The discrepancy between HRM and PCR-RFLP results was investigated using PCR-RFLP results available from our previous study.
resultsThe study included 354 women: 272 patients with POP and 82 controls; 18 (7 %) cases versus 3 (4 %) controls had a homozygous rs1800255, COL3A1 2209 G>A polymorphism (odds ratio 1.9, 95 % confidence interval 0.5-6.9, compared to the wild type), and thus no association between POP and the homozygous polymorphism could be demonstrated. A discrepancy between HRM and PCR-RFLP results was found in 8 % of the samples.
conclusionsThe previously found statistically significant association between the rs1800255, COL3A1 2209 G>A polymorphism as measured with PCR-RFLP and POP could no longer be demonstrated. This raises concerns regarding the results of other association studies using PCR-RFLP.
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