ArticlePloS one2014
Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Decidual CD4 Effector Memory and Resident Memory T Cells Respond to Cord Blood Allo-Antigens.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Article
- Human leukocyte antigen variation is associated with cytomegalovirus serostatus in healthy individuals.American journal of human genetics · 2025Article
- A vaccine against cytomegalovirus: how close are we?The Journal of clinical investigation · 2025Article
- Epitope mapping using immunopeptidomics reveals novel immunodominant CD8 T cell epitopes of the AAV9 capsid.Frontiers in immunology · 2025Article
- Identification of novel canonical and cryptic HCMV-specific T-cell epitopes for HLA-A∗03 and HLA-B∗15 via peptide-PRISM.Blood advances · 2024Article
- HLA Class II Specificity Assessed by High-Density Peptide Microarray Interactions.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Immunoinformatics: Predicting Peptide-MHC Binding.Annual review of biomedical data science · 2020Article
- Bright and Early: Inhibiting Human Cytomegalovirus by Targeting Major Immediate-Early Gene Expression or Protein Function.Viruses · 2020Review
- Cytomegalovirus-specific CD8+ T-cell responses are associated with arterial blood pressure in people living with HIV.PloS one · 2020Article
- Precision mouse models with expanded tropism for human pathogens.Nature biotechnology · 2019Article
- The combination of neoantigen quality and T lymphocyte infiltrates identifies glioblastomas with the longest survival.Communications biology · 2019Article
- The molecular landscape of glioma in patients with Neurofibromatosis 1.Nature medicine · 2019Article
- The Presence of a CMV Immunodominant Allele in the Recipient Is Associated With Increased Survival in CMV Positive Patients Undergoing Haploidentical Hematopoietic Stem Cell Transplantation.Frontiers in oncology · 2019Article
- Identification of the cognate peptide-MHC target of T cell receptors using molecular modeling and force field scoring.Molecular immunology · 2018Article
- Cytomegalovirus (CMV) Epitope-Specific CD4Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Immunodominant cytomegalovirus-specific CD8+ T-cell responses in sub-Saharan African populations.PloS one · 2017Article
- Accurate pan-specific prediction of peptide-MHC class II binding affinity with improved binding core identification.Immunogenetics · 2015Article
- The immune response to cytomegalovirus in allogeneic hematopoietic stem cell transplant recipients.Cellular and molecular life sciences : CMLS · 2015Review
- A Diverse Repertoire of CD4 T Cells Targets the Immediate-Early 1 Protein of Human Cytomegalovirus.Frontiers in immunology · 2015Article
- Automatic Generation of Validated Specific Epitope Sets.Journal of immunology research · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human cytomegalovirus (HCMV) is an important human pathogen. It is a leading cause of congenital infection and a leading infectious threat to recipients of solid organ transplants as well as of allogeneic hematopoietic cell transplants. Moreover, it has recently been suggested that HCMV may promote tumor development. Both CD4+ and CD8+ T cell responses are important for long-term control of the virus, and adoptive transfer of HCMV-specific T cells has led to protection from reactivation and HCMV disease. Identification of HCMV-specific T cell epitopes has primarily focused on CD8+ T cell responses against the pp65 phosphoprotein. In this study, we have focused on CD4+ and CD8+ T cell responses against the immediate early 1 and 2 proteins (IE1 and IE2). Using overlapping peptides spanning the entire IE1 and IE2 sequences, peripheral blood mononuclear cells from 16 healthy, HLA-typed, donors were screened by ex vivo IFN-γ ELISpot and in vitro intracellular cytokine secretion assays. The specificities of CD4+ and CD8+ T cell responses were identified and validated by HLA class II and I tetramers, respectively. Eighty-one CD4+ and 44 CD8+ T cell responses were identified representing at least seven different CD4 epitopes and 14 CD8 epitopes restricted by seven and 11 different HLA class II and I molecules, respectively, in total covering 91 and 98% of the Caucasian population, respectively. Presented in the context of several different HLA class II molecules, two epitope areas in IE1 and IE2 were recognized in about half of the analyzed donors. These data may be used to design a versatile anti-HCMV vaccine and/or immunotherapy strategy.
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