ArticleJournal of experimental botany2014
A novel gene, MdSSK1, as a component of the SCF complex rather than MdSBP1 can mediate the ubiquitination of S-RNase in apple.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- Pan-genome cloning and expression analysis of S-RNase homologous gene in SI and SC of different tomato species.Scientific reports · 2026Article
- Genome-wide identification and analysis ofFrontiers in plant science · 2026Article
- Comprehensive analysis of theForestry research · 2026Article
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- Molecular mechanisms and genetic regulation of self-incompatibility in flowering plants: implications for crop improvement and evolutionary biology.Plant molecular biology · 2025Review
- A regulatory network involving calmodulin controls phytosulfokine peptide processing during drought-induced flower abscission.The Plant cell · 2024Article
- The Mechanism of Ovule Abortion in Self-Pollinated 'Hanfu' Apple Fruits and Related Gene Screening.Plants (Basel, Switzerland) · 2024Article
- The Identification and Analysis of the Self-Incompatibility Pollen Determinant FactorPlants (Basel, Switzerland) · 2024Article
- The influence of the pollination compatibility type on the pistilFrontiers in genetics · 2024Article
- Ethylene enhances MdMAPK3-mediated phosphorylation of MdNAC72 to promote apple fruit softening.The Plant cell · 2023Article
- Analyses of Cullin1 homologs reveal functional redundancy in S-RNase-based self-incompatibility and evolutionary relationships in eudicots.The Plant cell · 2023Article
- Genome-wide identification of members of thePhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- ERF4 and MYB52 transcription factors play antagonistic roles in regulating homogalacturonan de-methylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2021Article
- Ubiquitination of SPlant physiology · 2020Article
- A mutation near the active site of S-RNase causes self-compatibility in S-RNase-based self-incompatible plants.Plant molecular biology · 2020Article
- In-depth Understanding ofInternational journal of molecular sciences · 2020Article
- Article
- Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia 'He Xie'.Journal of experimental botany · 2019Article
- Identification of a Novel SBP1-Containing SCFFrontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a core factor in S-RNase-based gametophytic self-incompatibility (GSI), the SCF (SKP1-Cullin1-F-box-Rbx1) complex (including pollen determinant SLF, S-locus-F-box) functions as an E3 ubiquitin ligase on non-self S-RNase. The SCF complex is formed by SKP1 bridging between SLF, CUL1, and Rbx1; however, it is not known whether an SCF complex lacking SKP1 can mediate the ubiquitination of S-RNase. Three SKP1-like genes from pollen were cloned based on the structural features of the SLF-interacting-SKP1-like (SSK) gene and the 'Golden Delicious' apple genome. These genes have a motif of five amino acids following the standard 'WAFE' at the C terminal and, in addition, contain eight sheets and two helices. All three genes were expressed exclusively in pollen. In the yeast two-hybrid and pull-down assays only one was found to interact with MdSFBB and MdCUL1, suggesting it is the SLF-interacting SKP1-like gene in apple which was named MdSSK1. In vitro experiments using MdSSK1, S2-MdSFBB1 (S2-Malus domestica S-locus-F-box brother) and MdCUL1 proteins incubated with S 2-RNase and ubiquitin revealed that the SCF complex ubiquitinylates S-RNase in vitro, while MdSBP1 (Malus domestica S-RNase binding protein 1) could not functionally replace MdSSK1 in the SCF complex in ubiquitinylating S-RNase. According to the above experiments, MdSBP1 is probably the only factor responsible for recognition with S-RNase, while not a component of the SCF complex, and an SCF complex containing MdSSK1 is required for mediating the ubiquitination of S-RNase.
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