Evidence map›Paper›PMID 24759717›Full record

ArticleJournal of clinical microbiology2014

Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

Janine T Bossé, Yanwen Li, Øystein Angen, Lucy A Weinert, Roy R Chaudhuri, Matthew T Holden, Susanna M Williamson, Duncan J Maskell, Alexander W Tucker, Brendan W Wren and 3 more

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Histopathological lesions ofVeterinary research forum : an international quarterly journal · 2023
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  6. Genetic Diversity ofVeterinary sciences · 2022
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  10. Rapid Detection and Typing ofFrontiers in veterinary science · 2021
    Article
  11. Review
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  14. Article
  15. Article
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  17. Article
  18. Article
  19. Immunoprotective Efficacy of SixFrontiers in microbiology · 2016
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Janine T BosséSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom.
Yanwen LiSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom.
Øystein AngenNorwegian Veterinary Institute, Oslo, Norway.
Lucy A WeinertDepartment of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
Roy R ChaudhuriDepartment of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
Matthew T HoldenThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Susanna M WilliamsonAnimal Health Veterinary Laboratories Agency (AHVLA), Rougham Hill, Suffolk, United Kingdom.
Duncan J MaskellThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Alexander W TuckerThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Brendan W WrenDepartment of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Andrew N RycroftDepartment of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hertfordshire, United Kingdom.
Paul R LangfordSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom p.langford@imperial.ac.uk.
BRaDP1T consortium

Funding

Biotechnology and Biological Sciences Research Council BB/G003203/1Biotechnology and Biological Sciences Research Council BB/G018553/1Biotechnology and Biological Sciences Research Council BB/G019177/1Biotechnology and Biological Sciences Research Council BB/G019274/1Biotechnology and Biological Sciences Research Council BB/G020744/1
6 · The paper itself

Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Indexed as

Actinobacillus InfectionsActinobacillus pleuropneumoniaeAnimalsDNA, BacterialDNA PrimersHumansMolecular Sequence DataMultiplex Polymerase Chain ReactionSequence Analysis, DNASerogroupDNA, BacterialDNA Primers

Identifiers

PMID24759717
PMCPMC4097740

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.