ArticleJournal of clinical microbiology2014
Isolation and identification of an Enterobacter cloacae strain producing a novel subtype of Shiga toxin type 1.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Ribosome-inactivating proteins in bacteria.Microbial genomics · 2026Review
- Algorithm and Software to Type Stx Operons Accurately from Assembled Genomic Sequence.Microorganisms · 2026Article
- Global population structure of Shiga toxin-producingMicrobial genomics · 2026Article
- Closed genome sequences of 39 Shiga toxin-producingMicrobiology resource announcements · 2025Article
- Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli.Scientific reports · 2025Article
- Shiga Toxin: Emerging Producer Strains, Prophylactic Approaches, and Application in Cancer Therapy.Journal of cancer prevention · 2024Review
- A Comprehensive Review on Shiga Toxin Subtypes and Their Niche-Related Distribution Characteristics in Shiga-Toxin-ProducingMicroorganisms · 2024Review
- Genetic and virulence characteristics of hybrid Shiga toxin-producing and atypical enteropathogenicFrontiers in microbiology · 2024Article
- Characterization of Atypical Shiga Toxin Gene Sequences and Description of Stx2j, a New Subtype.Journal of clinical microbiology · 2022Article
- Genomic Characteristics of Stx2e-ProducingPathogens (Basel, Switzerland) · 2021Article
- Revisiting Species Identification within the Enterobacter cloacae Complex by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.Microbiology spectrum · 2021Article
- Combined Action of Shiga Toxin Type 2 and Subtilase Cytotoxin in the Pathogenesis of Hemolytic Uremic Syndrome.Toxins · 2021Article
- Genome Analysis of a HistoricalFrontiers in microbiology · 2020Article
- Review
- High prevalence of non-O157 Shiga toxin-producing Escherichia coli in beef cattle detected by combining four selective agars.BMC microbiology · 2019Article
- Multidrug-ResistantFrontiers in microbiology · 2019Article
- The Evasive Enemy: Insights into the Virulence and Epidemiology of the Emerging Attaching and Effacing PathogenInfection and immunity · 2019Review
- Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype.Scientific reports · 2018Article
- Complete Genome Sequence of a Shiga Toxin-ProducingGenome announcements · 2017Article
- Contaminated Stream Water as Source for Escherichia coli O157 Illness in Children.Emerging infectious diseases · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here the isolation and identification of a Shiga toxin 1 (Stx1)-producing Enterobacter cloacae strain, M12X01451, from a human clinical specimen. The bacterial isolate was identified as E. cloacae using a polyphasic approach that included phenotypic, genetic, and proteomic analyses. The M12X01451 stx1 was sequenced, and the holotoxin was found to share only 87% amino acid sequence identity with the nearest Stx1 subtype reference sequence. Sequence analysis of the regions immediately flanking stx1 displayed similarities with bacteriophage-related sequences, suggesting a prophage origin. The stx1 gene was a stable element within the M12X01451 genome, as demonstrated by real-time PCR detection following successive subculturing of the bacterial isolate. Culture supernatant from M12X01451 was cytotoxic to Vero cells but was not neutralized by an anti-Stx1 monoclonal antibody. In addition, Stx1 from M12X01451 demonstrated limited antigenicity with two commercially available lateral flow immunoassays. The M12X01451 Stx represents a new Stx1 subtype based on the degree of sequence dissimilarity with Stx1 subtype reference sequences and its limited reactivity with anti-Stx1 antibodies.
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