ArticleJournal of clinical microbiology2014
Bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria species.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Antimicrobial treatment in invasive infections caused byFrontiers in medicine · 2024Pooled it
- Zoonotic bacterial pathogens in global fish production: transmission, resistance, and one health control strategies.Veterinary research communications · 2026Review
- HumanBiomolecules & biomedicine · 2026Review
- Feasibility of anaerobic co-digestion for biogas production from recycled paper industry sludge: optimization of mixing ratios and application in two-stage CSTR system design.Water science and technology : a journal of the International Association on Water Pollution Research · 2025Article
- Identification and Differentiation of Non-HemolyticMolecules (Basel, Switzerland) · 2025Article
- Mucosal infection with Tsukamurella species following nasal septum procedure: a rare case report.Annals of medicine and surgery (2012) · 2024Article
- Application of MALDI-TOF mass spectrometry for identification of Nocardia species.BMC microbiology · 2024Article
- Article
- Review
- Catheter-related bloodstream infection caused by Tsukamurella tyrosinosolvens identified by secA1sequencing in an immunocompromised child: a case report.Annals of clinical microbiology and antimicrobials · 2023Article
- Methods of IdentifyingPathogens (Basel, Switzerland) · 2022Review
- Serotype-identifying ions inHeliyon · 2022Article
- Gordonia bronchialis Bacteremia in a Patient With Burkitt Lymphoma: A Case Report and Literature Review.Cureus · 2022Article
- Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan.Scientific reports · 2021Article
- Article
- Evaluation of three sample preparation methods for the identification of clinical strains by using two MALDI-TOF MS systems.Journal of mass spectrometry : JMS · 2021Article
- Review of the impact of MALDI-TOF MS in public health and hospital hygiene, 2018.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) score algorithm for identification of Gordonia species.AMB Express · 2018Article
- Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species.Journal of clinical microbiology · 2018Article
- MALDI-TOF MS for identification of Tsukamurella species: Tsukamurella tyrosinosolvens as the predominant species associated with ocular infections.Emerging microbes & infections · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated whether the Bruker Biotyper matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system provides accurate species-level identifications of 147 isolates of aerobically growing Gram-positive rods (GPRs). The bacterial isolates included Nocardia (n = 74), Listeria (n = 39), Kocuria (n = 15), Rhodococcus (n = 10), Gordonia (n = 7), and Tsukamurella (n = 2) species, which had all been identified by conventional methods, molecular methods, or both. In total, 89.7% of Listeria monocytogenes, 80% of Rhodococcus species, 26.7% of Kocuria species, and 14.9% of Nocardia species (n = 11, all N. nova and N. otitidiscaviarum) were correctly identified to the species level (score values, ≥ 2.0). A clustering analysis of spectra generated by the Bruker Biotyper identified six clusters of Nocardia species, i.e., cluster 1 (N. cyriacigeorgica), cluster 2 (N. brasiliensis), cluster 3 (N. farcinica), cluster 4 (N. puris), cluster 5 (N. asiatica), and cluster 6 (N. beijingensis), based on the six peaks generated by ClinProTools with the genetic algorithm, i.e., m/z 2,774.477 (cluster 1), m/z 5,389.792 (cluster 2), m/z 6,505.720 (cluster 3), m/z 5,428.795 (cluster 4), m/z 6,525.326 (cluster 5), and m/z 16,085.216 (cluster 6). Two clusters of L. monocytogenes spectra were also found according to the five peaks, i.e., m/z 5,594.85, m/z 6,184.39, and m/z 11,187.31, for cluster 1 (serotype 1/2a) and m/z 5,601.21 and m/z 11,199.33 for cluster 2 (serotypes 1/2b and 4b). The Bruker Biotyper system was unable to accurately identify Nocardia (except for N. nova and N. otitidiscaviarum), Tsukamurella, or Gordonia species. Continuous expansion of the MALDI-TOF MS databases to include more GPRs is necessary.
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