ArticleScientific reports2014
Single cell genome analysis of an uncultured heterotrophic stramenopile.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.Molecular plant pathology · 2025Review
- MarFERReT, an open-source, version-controlled reference library of marine microbial eukaryote functional genes.Scientific data · 2023Article
- Genome sequencing and de novo assembly of the giant unicellular alga Acetabularia acetabulum using droplet MDA.Scientific reports · 2021Article
- Comparative genomics reveals new functional insights in uncultured MAST species.The ISME journal · 2021Article
- Article
- SCGid: a consensus approach to contig filtering and genome prediction from single-cell sequencing libraries of uncultured eukaryotes.Bioinformatics (Oxford, England) · 2020Article
- Unexpected mitochondrial genome diversity revealed by targeted single-cell genomics of heterotrophic flagellated protists.Nature microbiology · 2020Article
- Single Cell Genomics Reveals Viruses Consumed by Marine Protists.Frontiers in microbiology · 2020Article
- A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Combining morphology, behaviour and genomics to understand the evolution and ecology of microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Single cell ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- The need to account for cell biology in characterizing predatory mixotrophs in aquatic environments.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Entities inside one another - a matryoshka doll in biology?Environmental microbiology reports · 2019Article
- Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada).Frontiers in microbiology · 2019Article
- Leveraging single-cell genomics to expand the fungal tree of life.Nature microbiology · 2018Article
- Multigene phylogeny and cell evolution of chromist infrakingdom Rhizaria: contrasting cell organisation of sister phyla Cercozoa and Retaria.Protoplasma · 2018Article
- Genome-reconstruction for eukaryotes from complex natural microbial communities.Genome research · 2018Article
- Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans.Nature communications · 2018Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A broad swath of eukaryotic microbial biodiversity cannot be cultivated in the lab and is therefore inaccessible to conventional genome-wide comparative methods. One promising approach to study these lineages is single cell genomics (SCG), whereby an individual cell is captured from nature and genome data are produced from the amplified total DNA. Here we tested the efficacy of SCG to generate a draft genome assembly from a single sample, in this case a cell belonging to the broadly distributed MAST-4 uncultured marine stramenopiles. Using de novo gene prediction, we identified 6,996 protein-encoding genes in the MAST-4 genome. This genetic inventory was sufficient to place the cell within the ToL using multigene phylogenetics and provided preliminary insights into the complex evolutionary history of horizontal gene transfer (HGT) in the MAST-4 lineage.
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