ArticleInternational journal of molecular sciences2014
The lethal giant larvae gene in Tribolium castaneum: molecular properties and roles in larval and pupal development as revealed by RNA interference.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 17 citations in OpenAlex.
- First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.Insects · 2021Article
- Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis.Pest management science · 2021Article
- RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella.PloS one · 2021Article
- Molecular and Potential Regulatory Mechanisms of Melanin Synthesis inInternational journal of molecular sciences · 2020Article
- The Role of the Dopamine Melanin Pathway in the Ontogeny of Elytral Melanization inFrontiers in physiology · 2019Article
- Characterization and RNAi-mediated knockdown of Chitin Synthase A in the potato tuber moth, Phthorimaea operculella.Scientific reports · 2017Article
- RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum.Scientific reports · 2016Article
- Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).International journal of molecular sciences · 2015Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl) protein from the red flour beetle (Tribolium castaneum). Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl in insect development, RNA interference was performed in early (1-day) larvae, late (20-day) larvae, and early (1-day) pupae. The early larvae injected with double-stranded RNA of TcLgl (dsTcLgl) at 100, 200, and 400 ng/larva failed to pupate, and 100% mortality was achieved within 20 days after the injection or before the pupation. The late larvae injected with dsTcLgl at these doses reduced the pupation rates to only 50.3%, 36.0%, and 18.2%, respectively. The un-pupated larvae gradually died after one week, and visually unaffected pupae failed to emerge into adults and died during the pupal stage. Similarly, when early pupae were injected with dsTcLgl at these doses, the normal eclosion rates were reduced to only 22.5%, 18.0%, and 11.2%, respectively, on day 7 after the injection, and all the adults with abnormal eclosion died in two days after the eclosion. These results indicate that TcLgl plays an essential role in insect development, especially during their metamorphosis.
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