ArticleBMC plant biology2014
The Theobroma cacao B3 domain transcription factor TcLEC2 plays a duel role in control of embryo development and maturation.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Plant Regeneration: Influencing Factors, Regulatory Networks, and Epigenetic Mechanisms.International journal of molecular sciences · 2026Review
- Functional analysis of the woody oil crop Plukenetia volubilis L. LEC2 homolog PvoB3-69 in promoting regeneration.Plant cell reports · 2025Article
- Advances in Somatic Embryogenesis of Banana.International journal of molecular sciences · 2023Review
- Transcriptomic and metabolomic profiling reveals the drought tolerance mechanism ofFrontiers in plant science · 2023Article
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- Genome-wide analysis of the B3 transcription factors reveals that RcABI3/VP1 subfamily plays important roles in seed development and oil storage in castor bean (Plant diversity · 2022Article
- Transduction of Signals during Somatic Embryogenesis.Plants (Basel, Switzerland) · 2022Review
- Integrated transcriptomic and metabolic analyses provide insights into the maintenance of embryogenic potential and the biosynthesis of phenolic acids and flavonoids involving transcription factors inFrontiers in plant science · 2022Article
- ABA signalling promotes cell totipotency in the shoot apex of germinating embryos.Journal of experimental botany · 2021Article
- A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation.Frontiers in plant science · 2021Article
- Genes, proteins and other networks regulating somatic embryogenesis in plants.Journal, genetic engineering & biotechnology · 2020Review
- Genomic Organization of the B3-Domain Transcription Factor Family in Grapevine (International journal of molecular sciences · 2019Article
- Cocoa Bean Proteins-Characterization, Changes and Modifications due to Ripening and Post-Harvest Processing.Nutrients · 2019Review
- Signaling Overview of Plant Somatic Embryogenesis.Frontiers in plant science · 2019Review
- ArabidopsisFrontiers in plant science · 2019Article
- RNA sequencing and anthocyanin synthesis-related genes expression analyses in white-fruited Vaccinium uliginosum.BMC genomics · 2018Article
- Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development.Plant reproduction · 2018Review
- Cacao biotechnology: current status and future prospects.Plant biotechnology journal · 2018Review
- Glucocorticoid receptor-regulated TcLEC2 expression triggers somatic embryogenesis in Theobroma cacao leaf tissue.PloS one · 2018Article
- Profiling the onset of somatic embryogenesis in Arabidopsis.BMC genomics · 2017Article
Corrections and comments
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Arabidopsis thaliana LEC2 gene encodes a B3 domain transcription factor, which plays critical roles during both zygotic and somatic embryogenesis. LEC2 exerts significant impacts on determining embryogenic potential and various metabolic processes through a complicated genetic regulatory network.
resultsAn ortholog of the Arabidopsis Leafy Cotyledon 2 gene (AtLEC2) was characterized in Theobroma cacao (TcLEC2). TcLEC2 encodes a B3 domain transcription factor preferentially expressed during early and late zygotic embryo development. The expression of TcLEC2 was higher in dedifferentiated cells competent for somatic embryogenesis (embryogenic calli), compared to non-embryogenic calli. Transient overexpression of TcLEC2 in immature zygotic embryos resulted in changes in gene expression profiles and fatty acid composition. Ectopic expression of TcLEC2 in cacao leaves changed the expression levels of several seed related genes. The overexpression of TcLEC2 in cacao explants greatly increased the frequency of regeneration of stably transformed somatic embryos. TcLEC2 overexpressing cotyledon explants exhibited a very high level of embryogenic competency and when cultured on hormone free medium, exhibited an iterative embryogenic chain-reaction.
conclusionsOur study revealed essential roles of TcLEC2 during both zygotic and somatic embryo development. Collectively, our evidence supports the conclusion that TcLEC2 is a functional ortholog of AtLEC2 and that it is involved in similar genetic regulatory networks during cacao somatic embryogenesis. To our knowledge, this is the first detailed report of the functional analysis of a LEC2 ortholog in a species other then Arabidopsis. TcLEC2 could potentially be used as a biomarker for the improvement of the SE process and screen for elite varieties in cacao germplasm.
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