ArticleTranslational vision science & technology2014
Nonthermal Dielectric Barrier Discharge (DBD) Plasma Suppresses Herpes Simplex Virus Type 1 (HSV-1) Replication in Corneal Epithelium.
Article in Translational vision science & technology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Medical Gas Plasma Inactivates Adenoviruses via Capsid Oxidation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Treatment and Cleaning of Contact Lenses with Plasma-Activated Solutions.Pathogens (Basel, Switzerland) · 2026Article
- Effect of cold atmospheric plasma on common oral pathogenic microorganisms: a narrative review.Annals of medicine · 2025Review
- The effects of ex vivo physical cold atmospheric plasma treatment on endothelial cells in human corneal explants and its potential for in vivo application in refractory corneal ulcers.Scientific reports · 2025Article
- Herpes simplex virus type-1 infection and spread in a novel porcine corneal explant model is restricted to the epithelium.PLoS pathogens · 2025Article
- Immunological Control of Herpes Simplex Virus Type 1 Infection: A Non-Thermal Plasma-Based Approach.Viruses · 2025Review
- Biomimetic Hydrogels - Tools for Regenerative Medicine, Oncology, and Understanding Medical Gas Plasma Therapy.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Non-Thermal Plasma Reduces HSV-1 Infection of and Replication in HaCaT Keratinocytes In Vitro.International journal of molecular sciences · 2024Article
- Manipulation of Oxidative Stress Responses by Non-Thermal Plasma to Treat Herpes Simplex Virus Type 1 Infection and Disease.International journal of molecular sciences · 2023Review
- Argon cold atmospheric plasma eradicates pathogensFrontiers in veterinary science · 2023Article
- Oral SARS-CoV-2 reduction by local treatment: A plasma technology application?Plasma processes and polymers (Print) · 2022Article
- Safety evaluation of the atmospheric low-temperature plasma (ALTP) on the conjunctiva: an animal study and histopathological findings; 6-month follow-up.BMC ophthalmology · 2021Article
- Non-thermal plasma application enhances the recovery of transected sciatic nerves in rats.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Applications and challenges of low temperature plasma in pharmaceutical field.Journal of pharmaceutical analysis · 2021Review
- Cold Plasma, a New Hope in the Field of Virus Inactivation.Trends in biotechnology · 2020Review
- Gas Plasma Technology-An Asset to Healthcare During Viral Pandemics Such as the COVID-19 Crisis?IEEE transactions on radiation and plasma medical sciences · 2020Article
- Cold atmospheric plasma as antiviral therapy - effect on human herpes simplex virus type 1.The Journal of general virology · 2020Article
- Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machine.PloS one · 2020Article
- Key role of singlet oxygen and peroxynitrite in viral RNA damage during virucidal effect of plasma torch on feline calicivirus.Scientific reports · 2018Article
- Inactivation Efficacy of Nonthermal Plasma-Activated Solutions against Newcastle Disease Virus.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeHerpes keratitis (HK) is the leading cause of cornea-derived and infection-associated blindness in the developed world. Despite the availability of effective antivirals, some patients develop refractory disease, drug-resistant infection, and topical toxicity. A nonpharmaceutical treatment modality may offer a unique advantage in the management of such cases. This study investigated the antiviral effect of nonthermal dielectric barrier discharge (DBD) plasma, a partially ionized gas that can be applied to organic substances to produce various biological effects.
methodsHuman corneal epithelial cells and explanted corneas were infected with herpes simplex virus type 1 (HSV-1) and exposed to culture medium treated with nonthermal DBD plasma. The extent of infection was measured by plaque assay, quantitative PCR, and Western blot. Corneal toxicity assessment was performed with fluorescein staining, histologic examination, and 8-OHdG detection.
resultsApplication of DBD plasma-treated medium to human corneal epithelial cells and explanted corneas produced a dose-dependent reduction of the cytopathic effect, viral genome replication, and the overall production of infectious viral progeny. Toxicity studies showed lack of detrimental effects in explanted human corneas.
conclusionsNonthermal DBD plasma substantially suppresses corneal HSV-1 infection in vitro and ex vivo without causing pronounced toxicity. TRANSLATIONAL RELEVANCE: Nonthermal plasma is a versatile tool that holds great biomedical potential for ophthalmology, where it is being investigated for wound healing and sterilization and is already in use for ocular microsurgery. The anti-HSV-1 activity of DBD plasma demonstrated here could be directly translated to the clinic for use against drug-resistant herpes keratitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.