ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2014
Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 55 citations in OpenAlex.
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- Efficient Generation of CRISPR/Cas9-Mediated Homozygous/BiallelicFrontiers in plant science · 2020Article
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- Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare.Physiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture ofIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
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Authors and funding
4 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
An efficient hairy root induction system for an important endangered medicinal plant, Dracocephalum kotschyi, was developed through Agrobacterium rhizogenes-mediated transformation by modifying the co-cultivation medium using five bacterial strains, A4, ATCC15834, LBA9402, MSU440, and A13 (MAFF-02-10266). A drastic increase in transformation frequency was observed when a Murashige and Skoog medium lacking NH4NO3 KH2PO4, KNO3 and CaCl2 was used, resulting in hairy root induction frequencies of 52.3 %, 69.6 %, 48.6 %, 89.0 %, and 80.0 % by A4, A13, LBA9402, MSU440, and ATCC15834 strains, respectively. For shoot induction, hairy roots and unorganized tumors induced by strain ATCC15834 were placed on an MS media supplemented with 0.1, 0.25, 0.5, and 1 mg/l BA plus 0.1 mg/l NAA. The high frequency of shoot regeneration and number of shoot were obtained in the medium containing 0.25 mg/l BA and 0.1 mg/l NAA. Root induction occurred from the base of regenerated shoots on the MS medium supplemented with 0.5 mg/l IBA after 10 days.
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