ArticleProceedings of the National Academy of Sciences of the United States of America2014
Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slices.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Excitation-Dependent KThe journal of physical chemistry. A · 2025Article
- Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.Neurophotonics · 2025Article
- Two-Photon FRET/FLIM Imaging of Cerebral Neurons.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The interplay of intracellular calcium and zinc ions in response to electric field stimulation in primary rat cortical neuronsFrontiers in cellular neuroscience · 2023Article
- Mobile zinc as a modulator of sensory perception.FEBS letters · 2023Review
- Dissociated Hippocampal Neurons Exhibit Distinct ZnACS chemical neuroscience · 2020Article
- Copper signalling: causes and consequences.Cell communication and signaling : CCS · 2018Review
- Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.Brain structure & function · 2018Review
- Modelling zinc changes at the hippocampal mossy fiber synaptic cleft.Journal of computational neuroscience · 2016Article
- Activation of group II metabotropic glutamate receptors blocks zinc release from hippocampal mossy fibers.Biological research · 2014Article
- Lysosome-related organelles as mediators of metal homeostasis.The Journal of biological chemistry · 2014Review
- A Far-Red Emitting Probe for Unambiguous Detection of Mobile Zinc in Acidic Vesicles and Deep Tissue.Chemical scienceArticle
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Mossy fiber termini in the hippocampus accumulate Zn(2+), which is released with glutamate from synaptic vesicles upon neural excitation. Understanding the spatiotemporal regulation of mobile Zn(2+) at the synaptic level is challenging owing to the difficulty of visualizing Zn(2+) at individual synapses. Here we describe the use of zinc-responsive fluorescent probes together with two-photon microscopy to image Zn(2+) dynamics mediated by NMDA receptor-dependent long-term potentiation induction at single mossy fiber termini of dentate gyrus neurons in adult mouse hippocampal slices. The membrane-impermeant fluorescent Zn(2+) probe, 6-CO2H-ZAP4, was loaded into presynaptic vesicles in hippocampal mossy fiber termini upon KCl-induced depolarization, which triggers subsequent endocytosis and vesicular restoration. Local tetanic stimulation decreased the Zn(2+) signal observed at individual presynaptic sites, indicating release of the Zn(2+) from vesicles in synaptic potentiation. This synapse-level two-photon Zn(2+) imaging method enables monitoring of presynaptic Zn(2+) dynamics for improving the understanding of physiological roles of mobile Zn(2+) in regular and aberrant neurologic function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.