Evidence map›Paper›PMID 24755960›Full record

ArticleScientific reports2014

High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes.

Smita K Nair, Georgia D Tomaras, Ana Paula Sales, David Boczkowski, Cliburn Chan, Kelly Plonk, Yongting Cai, Jens Dannull, Thomas B Kepler, Scott K Pruitt and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Smita K Nair1] Departments of Surgery, Duke University Medical Center, Durham, NC 27710 [2].
Georgia D Tomaras1] Departments of Surgery, Duke University Medical Center, Durham, NC 27710 [2].
Ana Paula Sales1] Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27710 [2].
David BoczkowskiDepartments of Surgery, Duke University Medical Center, Durham, NC 27710.
Cliburn ChanBiostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27710.
Kelly PlonkDepartments of Surgery, Duke University Medical Center, Durham, NC 27710.
Yongting Cai1] Departments of Surgery, Duke University Medical Center, Durham, NC 27710 [2].
Jens DannullDepartments of Surgery, Duke University Medical Center, Durham, NC 27710.
Thomas B Kepler1] Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27710 [2].
Scott K Pruitt1] Departments of Surgery, Duke University Medical Center, Durham, NC 27710 [2].
Kent J WeinholdDepartments of Surgery, Duke University Medical Center, Durham, NC 27710.
Duke Medical Center · USDuke University Hospital · USDuke University · US

Funding

NIAID NIH HHS N01 AI040082
6 · The paper itself

Abstract

Emergence of drug-resistant strains of the pathogen Mycobacterium tuberculosis (Mtb) and the ineffectiveness of BCG in curtailing Mtb infection makes vaccine development for tuberculosis an important objective. Identifying immunogenic CD8+ T cell peptide epitopes is necessary for peptide-based vaccine strategies. We present a three-tiered strategy for identifying and validating immunogenic peptides: first, identify peptides that form stable complexes with class I MHC molecules; second, determine whether cytotoxic T lymphocytes (CTLs) raised against the whole protein antigen recognize and lyse target cells pulsed with peptides that passed step 1; third, determine whether peptides that passed step 2, when administered in vivo as a vaccine in HLA-A2 transgenic mice, elicit CTLs that lyse target cells expressing the whole protein antigen. Our innovative approach uses dendritic cells transfected with Mtb antigen-encoding mRNA to drive antigen expression. Using this strategy, we have identified five novel peptide epitopes from the Mtb proteins Apa, Mtb8.4 and Mtb19.

Indexed as

High-Throughput Screening AssaysAnimalsAntigen PresentationAntigens, BacterialDendritic CellsEpitope MappingEpitopes, T-LymphocyteHistocompatibility Antigens Class IMiceMice, TransgenicMycobacterium tuberculosisPeptidesProtein BindingT-Lymphocytes, CytotoxicT-Lymphocyte SubsetsTuberculosisAntigens, BacterialEpitopes, T-LymphocyteHistocompatibility Antigens Class IPeptides

Identifiers

PMID24755960
PMCPMC4894389
OpenAlexW2006440123

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.