ArticlePloS one2014
Transport of particles in intestinal mucus under simulated infant and adult physiological conditions: impact of mucus structure and extracellular DNA.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 92 citations in OpenAlex.
- Simulating the biochemical environment for infant intestinal digestion: key challenges and recommendations.Frontiers in nutrition · 2026Review
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives.Drug delivery and translational research · 2025Article
- Research Progress on the Application of Food Colloids in Precise Targeted Delivery of Drugs and Bioactive Compounds.Gels (Basel, Switzerland) · 2025Review
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Human mesofluidic intestinal model for studying transport of drug carriers and bacteria through a live mucosal barrier.Lab on a chip · 2025Article
- Using sodium glycodeoxycholate to develop a temporary infant-like gut barrier model,Frontiers in nutrition · 2025Article
- Porcine ex-vivo intestinal mucus has age-dependent blocking activity against transmissible gastroenteritis virus.Veterinary research · 2024Article
- A novel on-a-chip system with a 3D-bioinspired gut mucus suitable to investigate bacterial endotoxins dynamics.Materials today. Bio · 2024Article
- Computer modeling of digestive processes in the alimentary tract and their physiological regulation mechanisms: closing the gap between digestion models andFrontiers in nutrition · 2024Article
- Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.Advanced drug delivery reviews · 2023Review
- Review
- Effects of prophylactic antibiotics administration on barrier properties of intestinal mucosa and mucus from preterm born piglets.Drug delivery and translational research · 2023Article
- Mucus increases cell iron uptake to impact the release of pro-inflammatory mediators after particle exposure.Scientific reports · 2023Article
- Therapeutic antibodies - natural and pathological barriers and strategies to overcome them.Pharmacology & therapeutics · 2022Review
- Enhanced microscopic dynamics in mucus gels under a mechanical load in the linear viscoelastic regime.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health.EFSA journal. European Food Safety Authority · 2021Article
- Oral delivery of peptide therapeutics in infants: Challenges and opportunities.Advanced drug delivery reviews · 2021Review
- Multiple Particle Tracking Detects Changes in Brain Extracellular Matrix and Predicts Neurodevelopmental Age.ACS nano · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The final boundary between digested food and the cells that take up nutrients in the small intestine is a protective layer of mucus. In this work, the microstructural organization and permeability of the intestinal mucus have been determined under conditions simulating those of infant and adult human small intestines. As a model, we used the mucus from the proximal (jejunal) small intestines of piglets and adult pigs. Confocal microscopy of both unfixed and fixed mucosal tissue showed mucus lining the entire jejunal epithelium. The mucus contained DNA from shed epithelial cells at different stages of degradation, with higher amounts of DNA found in the adult pig. The pig mucus comprised a coherent network of mucin and DNA with higher viscosity than the more heterogeneous piglet mucus, which resulted in increased permeability of the latter to 500-nm and 1-µm latex beads. Multiple-particle tracking experiments revealed that diffusion of the probe particles was considerably enhanced after treating mucus with DNase. The fraction of diffusive 500-nm probe particles increased in the pig mucus from 0.6% to 64% and in the piglet mucus from ca. 30% to 77% after the treatment. This suggests that extracellular DNA can significantly contribute to the microrheology and barrier properties of the intestinal mucus layer. To our knowledge, this is the first time that the structure and permeability of the small intestinal mucus have been compared between different age groups and the contribution of extracellular DNA highlighted. The results help to define rules governing colloidal transport in the developing small intestine. These are required for engineering orally administered pharmaceutical preparations with improved delivery, as well as for fabricating novel foods with enhanced nutritional quality or for controlled calorie uptake.
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Registered trials
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