ArticleScientific reports2014
A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
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- Harnessing engineered symbionts to combat concurrent malaria and arboviruses transmission.Nature communications · 2025Article
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- Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei.Scientific reports · 2025Article
- Drug-induced ER stress leads to induction of programmed cell death pathways of the malaria parasite.Parasitology research · 2024Article
- Let it glow: genetically encoded fluorescent reporters in Plasmodium.Malaria journal · 2024Review
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- The claudin-like apicomplexan microneme protein is required for gliding motility and infectivity of Plasmodium sporozoites.PLoS pathogens · 2023Article
- The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites.PLoS pathogens · 2023Article
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- A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission.PLoS pathogens · 2022Article
- A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts.PLoS pathogens · 2022Article
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10 authors.
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Abstract
Experimental genetics have been widely used to explore the biology of the malaria parasites. The rodent parasites Plasmodium berghei and less frequently P. yoelii are commonly utilised, as their complete life cycle can be reproduced in the laboratory and because they are genetically tractable via homologous recombination. However, due to the limited number of drug-selectable markers, multiple modifications of the parasite genome are difficult to achieve and require large numbers of mice. Here we describe a novel strategy that combines positive-negative drug selection and flow cytometry-assisted sorting of fluorescent parasites for the rapid generation of drug-selectable marker-free P. berghei and P. yoelii mutant parasites expressing a GFP or a GFP-luciferase cassette, using minimal numbers of mice. We further illustrate how this new strategy facilitates phenotypic analysis of genetically modified parasites by fluorescence and bioluminescence imaging of P. berghei mutants arrested during liver stage development.
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