Evidence map›Paper›PMID 24755780›Full record

ArticleNature neuroscience2014

High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor.

François St-Pierre, Jesse D Marshall, Ying Yang, Yiyang Gong, Mark J Schnitzer, Michael Z Lin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 252 papers.

0numbers the graph read from it
0cells of the map it votes in
252citing papers in PubMed
23.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

252 citing papers in PubMed, 419 citations in OpenAlex.

  1. Review
  2. Article
  3. Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.bioRxiv : the preprint server for biology · 2026
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  4. Causal and directional elements of global brain dynamics.bioRxiv : the preprint server for biology · 2026
    Article
  5. Intrinsic space-time couplings governing multi-scale cortical dynamics.bioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. A Historical Outline of Fluorescence Spectroscopy in Ion Channel Research.Advances in experimental medicine and biology · 2026
    Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Genetically encoded sensors illuminateIBRO neuroscience reports · 2025
    Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Review
  20. Review

192 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

François St-Pierre1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Department of Pediatrics, Stanford University, Stanford, California, USA.
Jesse D Marshall1] James H. Clark Center, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Palo Alto, California, USA.
Ying Yang1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Department of Pediatrics, Stanford University, Stanford, California, USA.
Yiyang Gong1] James H. Clark Center, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Palo Alto, California, USA.
Mark J Schnitzer1] James H. Clark Center, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Palo Alto, California, USA. [3] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
Michael Z Lin1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Department of Pediatrics, Stanford University, Stanford, California, USA.
Stanford University · US

Funding

TRAINING & OUTREACH COREP50GM107615 · NIGMS · STANFORD UNIVERSITY · PI FERRELL, JAMES E. · 2013 to 2017
$10.9M
Howard Hughes Medical InstituteNIGMS NIH HHS P50 GM107615
6 · The paper itself

Abstract

Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of ∼ 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.

Indexed as

Fluorescent DyesAction PotentialsAnimalsCells, CulturedChickensCiona intestinalisFemaleHEK293 CellsHumansMiceMolecular Sequence DataNeuronsPregnancyRatsRats, Sprague-DawleyTime FactorsFluorescent Dyes

Identifiers

PMID24755780
PMCPMC4494739
OpenAlexW2062382534

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.