Evidence map›Paper›PMID 24755708›Full record

ArticleNature communications2014

Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors.

Yiyang Gong, Mark J Wagner, Jin Zhong Li, Mark J Schnitzer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.

0numbers the graph read from it
0cells of the map it votes in
118citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

118 citing papers in PubMed.

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  12. Tutorial on compressed ultrafast photography.Journal of biomedical optics · 2024
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58 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Yiyang Gong1] James H. Clark Center, Stanford University, Stanford, California 94305, USA [2] CNC Program, Stanford University, Palo Alto, California 94303, USA.
Mark J Wagner1] James H. Clark Center, Stanford University, Stanford, California 94305, USA [2] CNC Program, Stanford University, Palo Alto, California 94303, USA.
Jin Zhong Li1] James H. Clark Center, Stanford University, Stanford, California 94305, USA [2] CNC Program, Stanford University, Palo Alto, California 94303, USA.
Mark J Schnitzer1] James H. Clark Center, Stanford University, Stanford, California 94305, USA [2] CNC Program, Stanford University, Palo Alto, California 94303, USA [3] Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA.

Funding

Training Program in Basic NeuroscienceT32MH020016 · NIMH · STANFORD UNIVERSITY · PI Justin L Gardner, Merritt C Maduke · 1997 to 2026
$16.2M
Massively Parallel Brain ImagingDP1OD003560 · OD · STANFORD UNIVERSITY · PI SCHNITZER, MARK J · 2007 to 2011
$3.9M
Howard Hughes Medical InstituteNIH HHS DP1 OD003560NIMH NIH HHS T32 MH020016
6 · The paper itself

Abstract

Genetically encoded fluorescence voltage sensors offer the possibility of directly visualizing neural spiking dynamics in cells targeted by their genetic class or connectivity. Sensors of this class have generally suffered performance-limiting tradeoffs between modest brightness, sluggish kinetics and limited signalling dynamic range in response to action potentials. Here we describe sensors that use fluorescence resonance energy transfer (FRET) to combine the rapid kinetics and substantial voltage-dependence of rhodopsin family voltage-sensing domains with the brightness of genetically engineered protein fluorophores. These FRET-opsin sensors significantly improve upon the spike detection fidelity offered by the genetically encoded voltage sensor, Arclight, while offering faster kinetics and higher brightness. Using FRET-opsin sensors we imaged neural spiking and sub-threshold membrane voltage dynamics in cultured neurons and in pyramidal cells within neocortical tissue slices. In live mice, rates and optical waveforms of cerebellar Purkinje neurons' dendritic voltage transients matched expectations for these cells' dendritic spikes.

Indexed as

AnimalsBrainCell LineDiagnostic ImagingElectrophysiologyFluorescence Resonance Energy TransferHumansMaleMiceNeuronsOpsinsOpsins

Identifiers

PMID24755708
PMCPMC4247277

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.