Evidence map›Paper›PMID 24755512›Full record

ArticlePlant physiology2014

Contrasting Roles of the Apoplastic Aspartyl Protease APOPLASTIC, ENHANCED DISEASE SUSCEPTIBILITY1-DEPENDENT1 and LEGUME LECTIN-LIKE PROTEIN1 in Arabidopsis Systemic Acquired Resistance.

Heiko H Breitenbach, Marion Wenig, Finni Wittek, Lucia Jordá, Ana M Maldonado-Alconada, Hakan Sarioglu, Thomas Colby, Claudia Knappe, Marlies Bichlmeier, Elisabeth Pabst and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed, 1 pooled it
11.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed, 1 synthesis or guideline pooled it, 142 citations in OpenAlex.

  1. Pooled it
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21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Heiko H BreitenbachHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Marion WenigHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Finni WittekHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Lucia JordáHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Ana M Maldonado-AlconadaHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Hakan SariogluHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Thomas ColbyHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Claudia KnappeHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Marlies BichlmeierHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Elisabeth PabstHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
David MackeyHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
Jane E ParkerHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.).
A Corina VlotHelmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology (H.H.B., M.W., F.W., C.K., M.B., E.P., A.C.V.), and Research Unit Protein Science (H.S.), 85764 Neuherberg, Germany;Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions (L.J., J.E.P., A.C.V.) and Mass Spectrometry Unit (T.C.), 50829 Cologne, Germany;John Innes Centre, Norwich NR4 7UH, United Kingdom (A.M.M.-A.); andOhio State University, Department of Horticulture and Crop Science and Department of Molecular Genetics, Columbus, Ohio 43210 (D.M.) corina.vlot@helmholtz-muenchen.de.
Max Planck Institute for Plant Breeding Research · DEJohn Innes Centre · GBThe Ohio State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Systemic acquired resistance (SAR) is an inducible immune response that depends on ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1). Here, we show that Arabidopsis (Arabidopsis thaliana) EDS1 is required for both SAR signal generation in primary infected leaves and SAR signal perception in systemic uninfected tissues. In contrast to SAR signal generation, local resistance remains intact in eds1 mutant plants in response to Pseudomonas syringae delivering the effector protein AvrRpm1. We utilized the SAR-specific phenotype of the eds1 mutant to identify new SAR regulatory proteins in plants conditionally expressing AvrRpm1. Comparative proteomic analysis of apoplast-enriched extracts from AvrRpm1-expressing wild-type and eds1 mutant plants led to the identification of 12 APOPLASTIC, EDS1-DEPENDENT (AED) proteins. The genes encoding AED1, a predicted aspartyl protease, and another AED, LEGUME LECTIN-LIKE PROTEIN1 (LLP1), were induced locally and systemically during SAR signaling and locally by salicylic acid (SA) or its functional analog, benzo 1,2,3-thiadiazole-7-carbothioic acid S-methyl ester. Because conditional overaccumulation of AED1-hemagglutinin inhibited SA-induced resistance and SAR but not local resistance, the data suggest that AED1 is part of a homeostatic feedback mechanism regulating systemic immunity. In llp1 mutant plants, SAR was compromised, whereas the local resistance that is normally associated with EDS1 and SA as well as responses to exogenous SA appeared largely unaffected. Together, these data indicate that LLP1 promotes systemic rather than local immunity, possibly in parallel with SA. Our analysis reveals new positive and negative components of SAR and reinforces the notion that SAR represents a distinct phase of plant immunity beyond local resistance.

Identifiers

PMID24755512
PMCPMC4044859
OpenAlexW2121726080

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.