ArticleThe Plant cell2014
Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
- The diverse roles of host membranes in plant-microbe interactions.PLoS pathogens · 2026Article
- Plasma membrane CYBDOM proteins catalyse apoplastic AsA regeneration and interact with RbohD to activate autophagy and drought tolerance in plants.Nature plants · 2025Article
- Membrane nanodomains to shape plant cellular functions and signaling.The New phytologist · 2025Review
- Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway.Plant communications · 2024Article
- Functionality of Reactive Oxygen Species (ROS) in Plants: Toxicity and Control inPlants (Basel, Switzerland) · 2024Review
- Insights into Plant Sensory Mechanisms under Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Seasonal dynamics of cell-to-cell transport in angiosperm wood.Journal of experimental botany · 2024Article
- XYLEM CYSTEINE PEPTIDASE 1 and its inhibitor CYSTATIN 6 regulate pattern-triggered immunity by modulating the stability of the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D.The Plant cell · 2024Article
- Membrane Dynamics Regulated by Cytoskeleton in Plant Immunity.International journal of molecular sciences · 2023Review
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- Involvement of the Membrane Nanodomain Protein,International journal of molecular sciences · 2023Article
- A SNARE-like protein fromFrontiers in plant science · 2023Article
- Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis.Frontiers in plant science · 2023Article
- Protein-Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Reactive oxygen species signalling in plant stress responses.Nature reviews. Molecular cell biology · 2022Review
- Receptor for Activated C Kinase1B (OsRACK1B) Impairs Fertility in Rice through NADPH-Dependent HInternational journal of molecular sciences · 2022Article
- Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst.Plant physiology · 2022Article
- ROOT HAIR DEFECTIVE 2 vesicular delivery to the apical plasma membrane domain during Arabidopsis root hair development.Plant physiology · 2022Article
- Lipid Signaling Requires ROS Production to Elicit Actin Cytoskeleton Remodeling during Plant Innate Immunity.International journal of molecular sciences · 2022Article
- Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains.The Plant cell · 2022Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis thaliana respiratory burst oxidase homolog D (RbohD) functions as an essential regulator of reactive oxygen species (ROS). However, our understanding of the regulation of RbohD remains limited. By variable-angle total internal reflection fluorescence microscopy, we demonstrate that green fluorescent protein (GFP)-RbohD organizes into dynamic spots at the plasma membrane. These RbohD spots have heterogeneous diffusion coefficients and oligomerization states, as measured by photobleaching techniques. Stimulation with ionomycin and calyculin A, which activate the ROS-producing enzymatic activity of RbohD, increases the diffusion and oligomerization of RbohD. Abscisic acid and flg22 treatments also increase the diffusion coefficient and clustering of GFP-RbohD. Single-particle analysis in clathrin heavy chain2 mutants and a Flotillin1 artificial microRNA line demonstrated that clathrin- and microdomain-dependent endocytic pathways cooperatively regulate RbohD dynamics. Under salt stress, GFP-RbohD assembles into clusters and then internalizes into the cytoplasm. Dual-color fluorescence cross-correlation spectroscopy analysis further showed that salt stress stimulates RbohD endocytosis via membrane microdomains. We demonstrate that microdomain-associated RbohD spots diffuse at the membrane with high heterogeneity, and these dynamics closely relate to RbohD activity. Our results provide insight into the regulation of RbohD activity by clustering and endocytosis, which facilitate the activation of redox signaling pathways.
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Registered trials
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