Evidence map›Paper›PMID 24755442›Full record

ArticleBMC research notes2014

Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa.

Chin-Shang Ho, Huei-Chuan Shih, Ho-Yih Liu, Shau-Ting Chiu, Mei-Hui Chen, Li-Ping Ju, Ya-Zhu Ko, Yu-Shen Shih, Chaur-Tzuhn Chen, Tsai-Wen Hsu and 1 more

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Article in BMC research notes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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6citing papers in PubMed
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6 citing papers in PubMed.

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5 · Who and what money

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11 authors.

Chin-Shang Ho
Huei-Chuan Shih
Ho-Yih Liu
Shau-Ting Chiu
Mei-Hui Chen
Li-Ping Ju
Ya-Zhu Ko
Yu-Shen Shih
Chaur-Tzuhn Chen
Tsai-Wen HsuDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan. twhsu@tesri.gov.tw.
Yu-Chung Chiang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundKeteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed on the IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteen polymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeria davidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var. chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variation available for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage.

findingsPolymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP and magnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosity ranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable.

conclusionsThese polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct evolutionary units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.

Indexed as

Endangered SpeciesGenetic SpeciationMicrosatellite RepeatsAllelesBase SequenceBreedingConservation of Natural ResourcesDNA, PlantGenetic LociGenetic MarkersHeterozygoteMolecular Sequence DataPhylogenyPinaceaePolymorphism, GeneticTaiwanDNA, PlantGenetic Markers

Identifiers

PMID24755442
PMCPMC4001355

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.