ArticleeLife2014
Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- The PRP19 Ubiquitin Ligase, Standing at the Cross-Roads of mRNA Processing and Genome Stability.Cancers · 2022Review
- Transcription and splicing dynamics during earlyRNA (New York, N.Y.) · 2022Article
- Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo.Development (Cambridge, England) · 2021Article
- Nanopore long-read RNA-seq and absolute quantification delineate transcription dynamics in early embryo development of an insect pest.Scientific reports · 2021Article
- NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.RNA (New York, N.Y.) · 2020Article
- ADisease models & mechanisms · 2020Article
- Moonlighting in Mitosis: Analysis of the Mitotic Functions of Transcription and Splicing Factors.Cells · 2020Review
- Evolutionary Dynamics of the SKN-1 → MED → END-1,3 Regulatory Gene Cascade inG3 (Bethesda, Md.) · 2020Article
- Nucleotide excision repair genes shaping embryonic development.Open biology · 2019Review
- Early genome activation inGenome research · 2019Article
- Alternative polyadenylation coordinates embryonic development, sexual dimorphism and longitudinal growth in Xenopus tropicalis.Cellular and molecular life sciences : CMLS · 2019Article
- Co-expression network analysis identifies gonad- and embryo-associated protein modules in the sentinel species Gammarus fossarum.Scientific reports · 2019Article
- The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor.Parasites & vectors · 2018Article
- Regulatory principles governing the maternal-to-zygotic transition: insights from Drosophila melanogaster.Open biology · 2018Review
- Article
- Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes.Nucleic acids research · 2018Article
- The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization.EMBO reports · 2018Article
- Scallop genome reveals molecular adaptations to semi-sessile life and neurotoxins.Nature communications · 2017Article
- Exploring the Impact of Cleavage and Polyadenylation Factors on Pre-mRNA Splicing Across Eukaryotes.G3 (Bethesda, Md.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being splicing avoidance a possible explanation why most early zygotic genes are intronless. We isolated two mutant alleles for a subunit of the NTC/Prp19 complexes, which specifically impaired pre-mRNA splicing of early zygotic but not maternally encoded transcripts. We hypothesized that the requirements for pre-mRNA splicing efficiency were likely to vary during development. Ectopic maternal expression of an early zygotic pre-mRNA was sufficient to suppress its splicing defects in the mutant background. Furthermore, a small early zygotic transcript with multiple introns was poorly spliced in wild-type embryos. Our findings demonstrate for the first time the existence of a developmental pre-requisite for highly efficient splicing during Drosophila early embryonic development and suggest in highly proliferative tissues a need for coordination between cell cycle and gene architecture to ensure correct gene expression and avoid abnormally processed transcripts. DOI: http://dx.doi.org/10.7554/eLife.02181.001.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.