Evidence map›Paper›PMID 24753733›Full record

ArticleBiomicrofluidics2014

Microfluidic device capable of medium recirculation for non-adherent cell culture.

Angela R Dixon, Shrinidhi Rajan, Chuan-Hsien Kuo, Tom Bersano, Rachel Wold, Nobuyuki Futai, Shuichi Takayama, Geeta Mehta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Angela R DixonDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Shrinidhi RajanDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Chuan-Hsien KuoDepartment of Mechanical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA ; Mobility and Thermal Management Department, General Dynamics Land Systems, Sterling Heights, Michigan 48310, USA.
Tom BersanoGoogle, Inc., 1600 Amphitheatre Parkway Mountain View, California 94043, USA ; University of Michigan Comprehensive Cancer Center, Ann Arbor, Michigan 48109, USA.
Rachel WoldDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Nobuyuki FutaiDepartment of Mechanical Engineering, Shibaura Institute of Technology, 3-5-1 Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Shuichi TakayamaDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA ; Department of Macromolecular Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Geeta MehtaDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA ; Department of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.

Funding

Microfluidic Analysis of Oscillatory Signaling Pathways Using Phase LockingR01GM096040 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TAKAYAMA, SHUICHI · 2011 to 2014
$1.1M
NIGMS NIH HHS R01 GM096040
6 · The paper itself

Abstract

We present a microfluidic device designed for maintenance and culture of non-adherent mammalian cells, which enables both recirculation and refreshing of medium, as well as easy harvesting of cells from the device. We demonstrate fabrication of a novel microfluidic device utilizing Braille perfusion for peristaltic fluid flow to enable switching between recirculation and refresh flow modes. Utilizing fluid flow simulations and the human promyelocytic leukemia cell line, HL-60, non-adherent cells, we demonstrate the utility of this RECIR-REFRESH device. With computer simulations, we profiled fluid flow and concentration gradients of autocrine factors and found that the geometry of the cell culture well plays a key role in cell entrapping and retaining autocrine and soluble factors. We subjected HL-60 cells, in the device, to a treatment regimen of 1.25% dimethylsulfoxide, every other day, to provoke differentiation and measured subsequent expression of CD11b on day 2 and day 4 and tumor necrosis factor-alpha (TNF-α) on day 4. Our findings display perfusion sensitive CD11b expression, but not TNF-α build-up, by day 4 of culture, with a 1:1 ratio of recirculation to refresh flow yielding the greatest increase in CD11b levels. RECIR-REFRESH facilitates programmable levels of cell differentiation in a HL-60 non-adherent cell population and can be expanded to other types of non-adherent cells such as hematopoietic stem cells.

Identifiers

PMID24753733
PMCPMC3977789

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.