ArticleNucleic acids research2014
Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- The symmetric and asymmetric impacts of external debt on economic growth in Tunisia: evidence from linear and nonlinear ARDL models.SN business & economics · 2023Article
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering.Nature communications · 2020Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells.Nature chemical biology · 2020Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- "Second-generation" fluorogenic RNA-based sensors.Methods (San Diego, Calif.) · 2019Review
- RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.Angewandte Chemie (International ed. in English) · 2019Review
- Aptamer-enabled uptake of small molecule ligands.Scientific reports · 2018Article
- Development of a genetically encodable FRET system using fluorescent RNA aptamers.Nature communications · 2018Article
- Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.International journal of molecular sciences · 2017Review
- Making the Message Clear: Concepts for mRNA Imaging.ACS central science · 2017Review
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.Frontiers in microbiology · 2017Review
- Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells.Methods (San Diego, Calif.) · 2016Article
- Aptamers in analytics.The Analyst · 2016Review
- Evolution and characterization of a benzylguanine-binding RNA aptamer.Chemical communications (Cambridge, England) · 2016Article
- IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters.Methods in enzymology · 2016Article
- Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes.Frontiers in chemistry · 2016Review
- RNA Fluorescence with Light-Up Aptamers.Frontiers in chemistry · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands that are separately conjugated with one of the FRET pair, Cy3 and Cy5. The IMAGEtags were expressed in yeast from the GAL1, ADH1 or ACT1 promoters. Transcription from all three promoters was imaged in live cells and transcriptional increases from the GAL1 promoter were observed with time after adding galactose. Expression of the IMAGEtags did not affect cell proliferation or endogenous gene expression. Advantages of this method are that no foreign proteins are produced in the cells that could be toxic or otherwise influence the cellular response as they accumulate, the IMAGEtags are short lived and oxygen is not required to generate their signals. The IMAGEtag RNA reporter system provides a means of tracking changes in transcriptional activity in live cells and in real time.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.