Evidence map›Paper›PMID 24753287›Full record

ArticleThe journal of gene medicine

Optimizing cationic and neutral lipids for efficient gene delivery at high serum content.

Chia-Ling Chan, Kai K Ewert, Ramsey N Majzoub, Yeu-Kuang Hwu, Keng S Liang, Cecília Leal, Cyrus R Safinya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of gene medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Lipid nanoparticle topology regulates endosomal escape and delivery of RNA to the cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. A review of the tortuous path of nonviral gene delivery and recent progress.International journal of biological macromolecules · 2021
    Review
  12. Article
  13. DNA Vaccines-How Far From Clinical Use?International journal of molecular sciences · 2018
    Review
  14. Article
  15. An optimized gene transfection system in WERI-Rb1 cells.International journal of molecular medicine · 2017
    Article
  16. Review
  17. Article
  18. Cationic liposome-nucleic acid nanoparticle assemblies with applications in gene delivery and gene silencing.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chia-Ling Chan *Department of Materials, Department of Physics, and Molecular, Cellular & Developmental Biology Department, University of California at Santa Barbara, California 93106, USA.
Kai K Ewert *Department of Materials, Department of Physics, and Molecular, Cellular & Developmental Biology Department, University of California at Santa Barbara, California 93106, USA.
Ramsey N MajzoubDepartment of Materials, Department of Physics, and Molecular, Cellular & Developmental Biology Department, University of California at Santa Barbara, California 93106, USA.
Yeu-Kuang HwuInstitute of Physics, Academia Sinica, Taipei 11529, Taiwan.
Keng S LiangNational Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
Cecília LealDepartment of Materials, Department of Physics, and Molecular, Cellular & Developmental Biology Department, University of California at Santa Barbara, California 93106, USA.
Cyrus R SafinyaDepartment of Materials, Department of Physics, and Molecular, Cellular & Developmental Biology Department, University of California at Santa Barbara, California 93106, USA.

Funding

Structures of Lipid-DNA Nonviral Gene Delivery Systems 4R01GM059288 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SAFINYA, CYRUS R · 1999 to 2016
$4.2M
NIGMS NIH HHS NIH GM-59288NIGMS NIH HHS R01 GM059288
6 · The paper itself

Abstract

backgroundCationic liposome (CL)-DNA complexes are promising gene delivery vectors with potential application in gene therapy. A key challenge in creating CL-DNA complexes for application is that their transfection efficiency (TE) is adversely affected by serum. In particular, little is known about the effects of a high serum content on TE, even though this may provide design guidelines for application in vivo.

methodsWe prepared CL-DNA complexes in which we varied the neutral lipid [1,2-dioleoyl-sn-glycerophosphatidylcholine, glycerol-monooleate (GMO), cholesterol], the headgroup charge and chemical structure of the cationic lipid, and the ratio of neutral to cationic lipid; we then measured the TE of these complexes as a function of serum content and assessed their cytotoxicity. We tested selected formulations in two human cancer cell lines (M21/melanoma and PC-3/prostate cancer).

resultsIn the absence of serum, all CL-DNA complexes of custom-synthesized multivalent lipids show high TE. Certain combinations of multivalent lipids and neutral lipids, such as MVL5(5+)/GMO-DNA complexes or complexes based on the dendritic-headgroup lipid TMVLG3(8+) exhibited high TE both in the absence and presence of serum. Although their TE still dropped to a small extent in the presence of serum, it reached or surpassed that of benchmark commercial transfection reagents, particularly at a high serum content.

conclusionsTwo-component vectors (one multivalent cationic lipid and one neutral lipid) can rival or surpass benchmark reagents at low and high serum contents (up to 50%, v/v). We propose guidelines for optimizing the serum resistance of CL-DNA complexes based on a given cationic lipid.

Indexed as

Gene Transfer TechniquesBenzamidesCationsCell Line, TumorDNAEscherichia coliFatty Acids, MonounsaturatedGenetic TherapyHumansLipidsLiposomesPlasmidsQuaternary Ammonium CompoundsSerumSpermineTransfection1,2-dioleoyloxy-3-(trimethylammonium)propaneBenzamidesCationsDNAFatty Acids, MonounsaturatedLipidsLiposomesN1-(2-(1-((3-aminopropyl)amino)-4-(di-(3-aminopropyl)amino)butylcarboxamido)ethyl)-3,4-di-(oleyloxy)benzamideQuaternary Ammonium CompoundsSperminecationic liposomesgene deliveryglycerol monooleatemultivalent cationic lipidserum

Identifiers

PMID24753287
PMCPMC4051313

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.