ArticleBiotechnology and bioengineering2014
Inferring alterations in cell-to-cell communication in HER2+ breast cancer using secretome profiling of three cell models.
Article in Biotechnology and bioengineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- A robust strategy for proteomic identification of biomarkers of invasive phenotype complexed with extracellular heat shock proteins.Cell stress & chaperones · 2019Article
- Exploring the key communicator role of exosomes in cancer microenvironment through proteomics.Proteome science · 2019Review
- Exosomes: A Promising Avenue for the Diagnosis of Breast Cancer.Technology in cancer research & treatment · 2019Review
- Exosomes derived from B16F0 melanoma cells alter the transcriptome of cytotoxic T cells that impacts mitochondrial respiration.The FEBS journal · 2018Article
- The effect of tumor-derived exosomes on immune regulation and cancer immunotherapy.Future oncology (London, England) · 2017Review
- Melanoma exosomes deliver a complex biological payload that upregulates PTPN11 to suppress T lymphocyte function.Pigment cell & melanoma research · 2017Article
- Identifying causal networks linking cancer processes and anti-tumor immunity using Bayesian network inference and metagene constructs.Biotechnology progress · 2016Article
- Eavesdropping on altered cell-to-cell signaling in cancer by secretome profiling.Molecular & cellular oncology · 2016Article
- Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers.The Analyst · 2015Article
- Coupling enrichment methods with proteomics for understanding and treating disease.Proteomics. Clinical applications · 2015Review
- Breast Cancer-Derived Extracellular Vesicles: Characterization and Contribution to the Metastatic Phenotype.BioMed research international · 2015Review
- Enhancing the discovery and development of immunotherapies for cancer using quantitative and systems pharmacology: Interleukin-12 as a case study.Journal for immunotherapy of cancer · 2015Review
- The active tamoxifen metabolite endoxifen (4OHNDtam) strongly down-regulates cytokeratin 6 (CK6) in MCF-7 breast cancer cells.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Challenges in demonstrating durable clinical responses to molecular-targeted therapies have sparked a re-emergence in viewing cancer as an evolutionary process. In somatic evolution, cellular variants are introduced through a random process of somatic mutation and are selected for improved fitness through a competition for survival. In contrast to Darwinian evolution, cellular variants that are retained may directly alter the fitness competition. If cell-to-cell communication is important for selection, the biochemical cues secreted by malignant cells that emerge should be altered to bias this fitness competition. To test this hypothesis, we compared the proteins secreted in vitro by two human HER2+ breast cancer cell lines (BT474 and SKBR3) relative to a normal human mammary epithelial cell line (184A1) using a proteomics workflow that leveraged two-dimensional gel electrophoresis (2DE) and MALDI-TOF mass spectrometry. Supported by the 2DE secretome maps and identified proteins, the two breast cancer cell lines exhibited secretome profiles that were similar to each other and, yet, were distinct from the 184A1 secretome. Using protein-protein interaction and pathway inference tools for functional annotation, the results suggest that all three cell lines secrete exosomes, as confirmed by scanning electron microscopy. Interestingly, the HER2+ breast cancer cell line exosomes are enriched in proteins involved in antigen-processing and presentation and glycolytic metabolism. These pathways are associated with two of the emerging hallmarks of cancer: evasion of tumor immunosurveillance and deregulating cellular energetics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.