ArticlePhotosynthesis research2014
The carbon concentrating mechanism in Chlamydomonas reinhardtii: finding the missing pieces.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- EPOS1 is required for normal pyrenoid starch sheath formation and efficiency of the COThe New phytologist · 2026Article
- Sensitivity of Photosystem II to Photoinhibition inInternational journal of molecular sciences · 2026Article
- Highly Active Carbonic Anhydrase of the Thylakoid Lumen ofPlants (Basel, Switzerland) · 2024Article
- CAH3 fromCells · 2024Review
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Responses of macroalgae to COEcology and evolution · 2019Article
- Article
- Ion and metabolite transport in the chloroplast of algae: lessons from land plants.Cellular and molecular life sciences : CMLS · 2018Review
- Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead.International journal of molecular sciences · 2018Review
- Enhancing growth-relevant metabolic pathways ofRSC advances · 2018Article
- Article
- Pyrenoid loss in Chlamydomonas reinhardtii causes limitations in CO2 supply, but not thylakoid operating efficiency.Journal of experimental botany · 2017Article
- Identification and characterization of a solute carrier, CIA8, involved in inorganic carbon acclimation in Chlamydomonas reinhardtii.Journal of experimental botany · 2017Article
- The diversity of CO2-concentrating mechanisms in marine diatoms as inferred from their genetic content.Journal of experimental botany · 2017Article
- A robust protocol for efficient generation, and genomic characterization of insertional mutants ofPlant methods · 2017Article
- The ins and outs of CO2.Journal of experimental botany · 2016Article
- CCM8: the eighth international symposium on inorganic carbon uptake by aquatic photosynthetic organisms.Photosynthesis research · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The photosynthetic, unicellular green alga, Chlamydomonas reinhardtii, lives in environments that often contain low concentrations of CO2 and HCO3 (-), the utilizable forms of inorganic carbon (Ci). C. reinhardtii possesses a carbon concentrating mechanism (CCM) which can provide suitable amounts of Ci for growth and development. This CCM is induced when the CO2 concentration is at air levels or lower and is comprised of a set of proteins that allow the efficient uptake of Ci into the cell as well as its directed transport to the site where Rubisco fixes CO2 into biomolecules. While several components of the CCM have been identified in recent years, the picture is still far from complete. To further improve our knowledge of the CCM, we undertook a mutagenesis project where an antibiotic resistance cassette was randomly inserted into the C. reinhardtii genome resulting in the generation of 22,000 mutants. The mutant collection was screened using both a published PCR-based approach (Gonzalez-Ballester et al. 2011) and a phenotypic growth screen. The PCR-based screen did not rely on a colony having an altered growth phenotype and was used to identify colonies with disruptions in genes previously identified as being associated with the CCM-related gene. Eleven independent insertional mutations were identified in eight different genes showing the usefulness of this approach in generating mutations in CCM-related genes of interest as well as identifying new CCM components. Further improvements of this method are also discussed.
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