ArticlePloS one2014
An engineered palette of metal ion quenchable fluorescent proteins.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- tKeima: A Large-Stokes-Shift Platform for Metal Ion Detection.Biosensors · 2026Article
- A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane.Structure (London, England : 1993) · 2026Article
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Structure-Based Understanding of CuBiosensors · 2025Review
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography.Journal of applied crystallography · 2023Article
- Peptide Self-Assembled Nanocarriers for Cancer Drug Delivery.The journal of physical chemistry. B · 2023Review
- Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper inACS sensors · 2022Article
- Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.Molecules (Basel, Switzerland) · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Spectroscopic Analysis of Fe Ion-Induced Fluorescence Quenching of the Green Fluorescent Protein ZsGreen.Journal of fluorescence · 2021Article
- Chromophorylation of a Novel Cyanobacteriochrome GAF Domain fromJournal of microbiology and biotechnology · 2021Article
- Spectroscopic and Structural Analysis of CuBiosensors · 2020Article
- Spectroscopic Analysis of the CuMolecular biotechnology · 2018Article
- The Dark Matter of Biology.Biophysical journal · 2016Review
- A novel copper-chelating strategy for fluorescent proteins to image dynamic copper fluctuations on live cell surfaces.Chemical science · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Many fluorescent proteins have been created to act as genetically encoded biosensors. With these sensors, changes in fluorescence report on chemical states in living cells. Transition metal ions such as copper, nickel, and zinc are crucial in many physiological and pathophysiological pathways. Here, we engineered a spectral series of optimized transition metal ion-binding fluorescent proteins that respond to metals with large changes in fluorescence intensity. These proteins can act as metal biosensors or imaging probes whose fluorescence can be tuned by metals. Each protein is uniquely modulated by four different metals (Cu2+, Ni2+, Co2+, and Zn2+). Crystallography revealed the geometry and location of metal binding to the engineered sites. When attached to the extracellular terminal of a membrane protein VAMP2, dimeric pairs of the sensors could be used in cells as ratiometric probes for transition metal ions. Thus, these engineered fluorescent proteins act as sensitive transition metal ion-responsive genetically encoded probes that span the visible spectrum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.