ArticlePloS one2014
The differentiation of human adipose-derived stem cells towards a urothelium-like phenotype in vitro and the dynamic temporal changes of related cytokines by both paracrine and autocrine signal regulation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Tissue engineering of urethra: Systematic review of recent literature.Experimental biology and medicine (Maywood, N.J.) · 2017Pooled it
- Use of adipose-derived stem cells on decellularized bladder scaffolds for functional bladder mucosa regeneration.Stem cells translational medicine · 2025Article
- Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells.Frontiers in bioengineering and biotechnology · 2025Article
- Tissue-engineered tubular substitutions for urinary diversion in a preclinical rabbit model.Frontiers in medicine · 2025Article
- Cell-Based Therapy for Urethral Regeneration: A Narrative Review and Future Perspectives.Biomedicines · 2023Review
- 3D Tumor Models in Urology.International journal of molecular sciences · 2023Review
- Sources, Selection, and Microenvironmental Preconditioning of Cells for Urethral Tissue Engineering.International journal of molecular sciences · 2022Review
- The urothelium: a multi-faceted barrier against a harsh environment.Mucosal immunology · 2022Review
- Tissue Engineering of the Urethra: From Bench to Bedside.Biomedicines · 2021Review
- Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering.International journal of molecular sciences · 2021Article
- Article
- Hypoxic Preconditioning Enhances Cellular Viability and Pro-angiogenic Paracrine Activity: The Roles of VEGF-A and SDF-1a in Rat Adipose Stem Cells.Frontiers in cell and developmental biology · 2020Article
- Regenerative and engineered options for urethroplasty.Nature reviews. Urology · 2019Review
- Review
- Differentiate into urothelium and smooth muscle cells from adipose tissue-derived stem cells for ureter reconstruction in a rabbit model.American journal of translational research · 2016Article
- Tissue-engineered tubular substitutions for urinary diversion in a rabbit model.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Production of urothelium from pluripotent stem cells for regenerative applications.Current urology reports · 2015Review
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the differentiation ability of human adipose-derived stem cells (ASCs) towards urothelium-like cells in vitro and the dynamic changes of related cytokines and cytokine receptors in the culture medium. MATERIALS AND
methodsThe ASCs were induced using both conditioned media (CM) and the transwell co-culture system with an immortalized urothelium cell line (SV-HUC-1,HUC) for 21 days. Protein and mRNA expression of the mature urothelium specific markers uroplakin-IA (UP-1A) and uroplakin-II (UP-II) were detected by immunofluorescence and quantitative real-time PCR, respectively. Array detection was used to screen 41 cytokines and receptors in the upper medium of urothelium, non-induced ASCs and urothelium-induced ASCs at three time points, early (12 hours), intermediate (7 days) and late (21 days).
resultsAfter induction for 7 days, the ASCs grown in both CM and transwell co-culture system expressed uroplakin-IA (13.54±2.00%; 17.28±1.84%) and uroplakin-II (19.49±1.73%; 13.98±1.47%). After induction for 21 days, ASCs grown in co-culture had significantly increased expression of uroplakin-IA (48.03±1.25%; 49.57±2.85%) and uroplakin-II (45.38±2.50%; 46.58±1.95%). In the upper medium of urothelium, 28 cytokines and 8 cytokine receptors had significantly higher expression than the counterpart of non-induced ASCs. After 7 days induction, the expression of 22 cytokines and 8 cytokine receptors was significantly elevated in the upper medium of induced ASCs compared to non-induced ASCs. At the early and intermediate time points, ASCs secreted high levels of relative cytokines and soluble receptors, but their expressions decreased significantly at the late time point.
conclusionThe adipose-derived stem cells have the potential to be differentiated into urothelium-like cells in vitro by both CM and transwell co-culture system with mature urothelium. Numerous cytokines and receptors were involved in the differentiation process with dynamic temporal changes by both paracrine and autocrine signal regulation. Further studies should be carried out to determine the detailed mechanism of cytokines and receptors and to enhance the urothelium differentiation efficiency of ASCs.
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