ArticlePloS one2014
Birth of healthy offspring following ICSI in in vitro-matured common marmoset (Callithrix jacchus) oocytes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Recovery of high-quality sperm after cryopreservation in the common marmoset (Callithrix jacchus)†.Biology of reproduction · 2026Article
- A chorionic gonadotropin assay enables non-invasive detection of ovulation and early pregnancy in a New World primate model.bioRxiv : the preprint server for biology · 2026Article
- The role of emerging assisted reproductive technologies in the conservation of neotropical wild mammals - challenges, advances and perspectives.The Journal of reproduction and development · 2026Review
- Article
- Derivation of embryonic stem cells from cloned blastocysts using improved somatic cell nuclear transfer in common marmosets.Stem cell reports · 2025Article
- Article
- Prevalence of major diseases in common marmosets (Frontiers in veterinary science · 2025Article
- Production of a heterozygous exon skipping model of common marmosets using gene-editing technology.Lab animal · 2024Article
- Generation of marmoset primordial germ cell-like cells under chemically defined conditions.Life science alliance · 2024Article
- In vitro matured oocytes have a higher developmental potential than in vivo matured oocytes after hormonal ovarian stimulation in Callithrix jacchus.Journal of ovarian research · 2024Article
- Early molecular events of autosomal-dominant Alzheimer's disease in marmosets with PSEN1 mutations.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2024Article
- Production of marmoset eggs and embryos from xenotransplanted ovary tissues.Scientific reports · 2023Article
- Optimization of piggyBac transposon-mediated gene transfer method in common marmoset embryos.PloS one · 2023Article
- Ultrasonography of the neotropical primate female reproductive system.Frontiers in veterinary science · 2023Review
- Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis).The Journal of reproduction and development · 2021Article
- Comparative computer-assisted sperm analysis in non-human primates.Journal of medical primatology · 2021Article
- Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.ILAR journal · 2020Review
- The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets.Scientific reports · 2020Article
- Novel gastrointestinal disease in common marmosets characterised by duodenal dilation: a clinical and pathological study.Scientific reports · 2020Article
- In Vitro Culture of Embryos from the Common Marmoset (Callithrix jacchus).Methods in molecular biology (Clifton, N.J.) · 2019Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ICSI. To investigate the optimal timing of performing ICSI, corrected immature oocytes were matured in vitro and ICSI was performed at various time points (1-2 h, 2-4 h, 4-6 h, 6-8 h, and 8-10 h after extrusion of the first polar body (PB)). Matured oocytes were then divided randomly into two groups: one was used for in vitro fertilization (IVF) and the other for ICSI. To investigate in vivo development of embryos followed by ICSI, 6-cell- to 8-cell-stage embryos and blastocysts were nonsurgically transferred into recipient marmosets. Although no significant differences were observed in the fertilization rate of blastocysts among ICSI timing after the first PB extrusion, the blastocyst rate at 1-2 h was lowest among groups at 2-4 h, 4-6 h, 6-8 h, and 8-10 h. Comparing ICSI to IVF, the fertilization rates obtained in ICSI were higher than in IVF (p>0.05). No significant difference was noted in the cleaved blastocyst rate between ICSI and IVF. Following the transfer of 37 ICSI blastocysts, 4 of 20 recipients became pregnant, while with the transfer of 21 6-cell- to 8-cell-stage ICSI embryos, 3 of 8 recipients became pregnant. Four healthy offspring were produced and grew normally. These are the first marmoset offspring produced by ICSI, making it an effective fertilization method for marmosets.
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