ArticleJournal of applied microbiology2014
Synthesis of conjugated linoleic acid by the linoleate isomerase complex in food-derived lactobacilli.
Article in Journal of applied microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Screening, Physiological Characterization, Genomic Analysis and Optimization by Conjugated Linoleic Acid Bioconversion of TwoFoods (Basel, Switzerland) · 2026Article
- A Comprehensive Review on Medium- and Long-Chain Fatty Acid-Derived Metabolites: From Energy Sources to Metabolic Signals.Metabolites · 2026Review
- Article
- Biosynthesis of conjugated linoleic acid: current status and future perspectives.Bioresources and bioprocessing · 2025Review
- Conjugated linoleic acid metabolite impact in colorectal cancer: a potential microbiome-based precision nutrition approach.Nutrition reviews · 2025Review
- Conjugated Linoleic Acid in Cancer Therapy.Current drug delivery · 2025Review
- Potential strategies to enhance conjugated linoleic acid content of milk and dairy products: A review.Heliyon · 2024Review
- Production of Conjugated Linoleic Acid (CLA) byFoods (Basel, Switzerland) · 2024Review
- Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans.Animals : an open access journal from MDPI · 2023Review
- Expression of heterologous heparan sulphate binding protein of3 Biotech · 2023Article
- The Mechanisms of the Potential ProbioticFoods (Basel, Switzerland) · 2022Review
- The Arginine Repressor ArgRMicrobiology spectrum · 2022Article
- Effect of Coix Seed Extracts on Growth and Metabolism ofFoods (Basel, Switzerland) · 2022Article
- Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated fromMicroorganisms · 2021Article
- Physicochemical Properties and Sensory Acceptability of a Next-Generation Functional Chocolate Added with Omega-3 Polyunsaturated Fatty Acids and Probiotics.Foods (Basel, Switzerland) · 2021Article
- Alleviation effects of Bifidobacterium breve on DSS-induced colitis depends on intestinal tract barrier maintenance and gut microbiota modulation.European journal of nutrition · 2021Article
- Physiological Characteristics ofJournal of microbiology and biotechnology · 2021Article
- Selective Isolation ofFrontiers in microbiology · 2021Article
- Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples.Asian-Australasian journal of animal sciences · 2020Article
- Letter to editor.Gut microbes · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
aimsTo assess strains of lactobacilli for their capacity to produce functional fatty acid-conjugated linoleic acid. To assess the linoleate isomerase for CLA production in the most efficient CLA producer. METHODS AND
resultsIn this study, strains of food-derived lactobacilli were cultured in media with linoleic acid and CLA production was assessed. Most of the selected strains produced CLA at different levels, with Lactobacillus plantarum ZS2058 being the most efficient CLA producer converting over 50% of linoleic acid to c9, t11-CLA and t9, t11-CLA. Some intermediates 10-hydroxy-cis-12-octadecenoic acid, 10-oxo-cis-12-octadecenoic acid and 10-oxo-trans-11-octadecenoic acid were determined via GC-MS. The genes coding the multicomponent linoleate isomerase containing myosin-cross-reactive antigen, short-chain dehydrogenase/oxidoreductase and acetoacetate decarboxylase for CLA production in Lact. plantarum ZS2058 were cloned and expressed in Escherichia coli. With the mixture of recombinant E. coli, c9, t11-CLA and three kinds of intermediates were produced from linoleic acid, which were in line with those in the lactobacilli.
conclusionsThe ability for CLA production by lactobacilli exhibited variation. Lactobacillus plantarum and Lact. bulgaricus were the most efficient producers in the selected strains. Lact. plantarum ZS2058 converted linoleic acid to CLAs with 10-hydroxy-cis-12-octadecenoic acid, 10-oxo-cis-12-octadecenoic acid and 10-oxo-trans-11-octadecenoic acid as intermediates. The multiple-step reactions for CLA production catalysed by multicomponent linoleate isomerase in Lact. plantarum ZS2058 were confirmed successfully. SIGNIFICANCE AND IMPACT OF THE STUDY: Multicomponent linoleate isomerase provides important results for the illustration of the mechanism for CLA production in lactic acid bacteria. Food-derived lactobacilli with CLA production ability offers novel opportunities for functional foods development.
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