ArticleMolecular microbiology2014
A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications.Nucleic acids research · 2025Article
- tRNA binding to Kti12 is crucial for wobble uridine modification by Elongator.Nucleic acids research · 2025Article
- Cryo-EM structures of the human Elongator complex at work.Nature communications · 2024Article
- Cryo-EM structure of the fully assembled Elongator complex.Nucleic acids research · 2023Article
- How Elongator Acetylates tRNA Bases.International journal of molecular sciences · 2020Review
- Molecular basis of tRNA recognition by the Elongator complex.Science advances · 2019Article
- Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator.Nucleic acids research · 2019Article
- Roles of Elongator Dependent tRNA Modification Pathways in Neurodegeneration and Cancer.Genes · 2018Review
- Structural insights into the function of Elongator.Cellular and molecular life sciences : CMLS · 2018Review
- Use of a Yeast tRNase Killer Toxin to Diagnose Kti12 Motifs Required for tRNA Modification by Elongator.Toxins · 2017Article
- Wobble uridine modifications-a reason to live, a reason to die?!RNA biology · 2017Review
- Architecture of the yeast Elongator complex.EMBO reports · 2017Article
- Dimerization of elongator protein 1 is essential for Elongator complex assembly.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.PLoS genetics · 2015Article
- Elongator, a conserved complex required for wobble uridine modifications in eukaryotes.RNA biology · 2014Review
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3 authors at 1 institution in 1 country.
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Abstract
Elongator is a conserved, multi-protein complex discovered in Saccharomyces cerevisiae, loss of which confers a range of pleiotropic phenotypes. Elongator in higher eukaryotes is required for normal growth and development and a mutation in the largest subunit of human Elongator (Elp1) causes familial dysautonomia, a severe recessive neuropathy. Elongator promotes addition of mcm(5) and ncm(5) modifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher eukaryotes. Since these modifications are required for the tRNAs to function efficiently, a translation defect caused by hypomodified tRNAs may therefore underlie the variety of phenotypes associated with Elongator dysfunction. The Elp1 carboxy-terminal domain contains a highly conserved arginine/lysine-rich region that resembles a nuclear localization sequence (NLS). Using alanine substitution mutagenesis, we show that this region is essential for Elongator's function in tRNA wobble uridine modification. However, rather than acting to determine the nucleo-cytoplasmic distribution of Elongator, we find that the basic region plays a critical role in a novel interaction between tRNA and the Elp1 carboxy-terminal domain. Thus the conserved basic region in Elp1 may be essential for tRNA wobble uridine modification by acting as tRNA binding motif.
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