ArticleInterface focus2014
Impact of heating on passive and active biomechanics of suspended cells.
Article in Interface focus, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.FEBS letters · 2025Review
- Sounding a New Era in Biomechanics with Acoustic Force Spectroscopy.Advances in experimental medicine and biology · 2023Article
- Thermal stress involved in TRPV2 promotes tumorigenesis through the pathways of HSP70/27 and PI3K/Akt/mTOR in esophageal squamous cell carcinoma.British journal of cancer · 2022Article
- PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers.Materials advances · 2022Article
- Cell mechanical properties of human breast carcinoma cells depend on temperature.Scientific reports · 2021Article
- Environmentally controlled magnetic nano-tweezer for living cells and extracellular matrices.Scientific reports · 2020Article
- Mechanical characterization of vesicles and cells: A review.Electrophoresis · 2020Review
- The Role of the Optical Stretcher Is Crucial in the Investigation of Cell Mechanics Regulating Cell Adhesion and Motility.Frontiers in cell and developmental biology · 2019Review
- Mechanical phenotyping of K562 cells by the Micropipette Aspiration Technique allows identifying mechanical changes induced by drugs.Scientific reports · 2018Article
- Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase.Biophysical journal · 2017Article
- Neutrophil recruitment limited by high-affinity bent β2 integrin binding ligand in cis.Nature communications · 2016Article
- A Comprehensive Review of Optical Stretcher for Cell Mechanical Characterization at Single-Cell Level.Micromachines · 2016Review
- Investigation of temperature effect on cell mechanics by optofluidic microchips.Biomedical optics express · 2015Article
- Directional bleb formation in spherical cells under temperature gradient.Biophysical journal · 2015Article
- Myosin II Activity Softens Cells in Suspension.Biophysical journal · 2015Article
- Article
- High-Throughput Assessment of Cellular Mechanical Properties.Annual review of biomedical engineering · 2015Review
- Cell mechanics: principles, practices, and prospects.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cell is a complex material whose mechanical properties are essential for its normal functions. Heating can have a dramatic effect on these mechanical properties, similar to its impact on the dynamics of artificial polymer networks. We investigated such mechanical changes by the use of a microfluidic optical stretcher, which allowed us to probe cell mechanics when the cells were subjected to different heating conditions at different time scales. We find that HL60/S4 myeloid precursor cells become mechanically more compliant and fluid-like when subjected to either a sudden laser-induced temperature increase or prolonged exposure to higher ambient temperature. Above a critical temperature of 52 ± 1°C, we observed active cell contraction, which was strongly correlated with calcium influx through temperature-sensitive transient receptor potential vanilloid 2 (TRPV2) ion channels, followed by a subsequent expansion in cell volume. The change from passive to active cellular response can be effectively described by a mechanical model incorporating both active stress and viscoelastic components. Our work highlights the role of TRPV2 in regulating the thermomechanical response of cells. It also offers insights into how cortical tension and osmotic pressure govern cell mechanics and regulate cell-shape changes in response to heat and mechanical stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.