ArticleJournal of ginseng research2014
Evidence of genome duplication revealed by sequence analysis of multi-loci expressed sequence tag-simple sequence repeat bands in Panax ginseng Meyer.
Article in Journal of ginseng research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Transcriptome-wide identification and systematic analysis of the Retrotransposon genes and their roles in ginsenoside biosynthesis in Panax ginseng.BMC plant biology · 2025Article
- High-resolution genetic map and SNP chip for molecular breeding inHorticulture research · 2024Article
- Genetic diversity among cultivated and wildJournal of ginseng research · 2020Article
- Comprehensive comparative analysis of chloroplast genomes from sevenJournal of ginseng research · 2020Article
- Phylogenomics and barcoding of Panax: toward the identification of ginseng species.BMC evolutionary biology · 2018Article
- Positive Selection Driving Cytoplasmic Genome Evolution of the Medicinally Important Ginseng Plant Genus Panax.Frontiers in plant science · 2018Article
- Evolution of the Araliaceae family inferred from complete chloroplast genomes and 45S nrDNAs of 10 Panax-related species.Scientific reports · 2017Article
- Discrimination of Korean ginseng (Journal of ginseng research · 2016Article
- The complete chloroplast genome sequence of a Korean indigenous ornamental plantMitochondrial DNA. Part B, Resources · 2016Article
- The impacts of polyploidy, geographic and ecological isolations on the diversification of Panax (Araliaceae).BMC plant biology · 2015Article
- Comprehensive Survey of Genetic Diversity in Chloroplast Genomes and 45S nrDNAs within Panax ginseng Species.PloS one · 2015Article
- Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products.Journal of ginseng research · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPanax ginseng, the most famous medicinal herb, has a highly duplicated genome structure. However, the genome duplication of P. ginseng has not been characterized at the sequence level. Multiple band patterns have been consistently observed during the development of DNA markers using unique sequences in P. ginseng.
methodsWe compared the sequences of multiple bands derived from unique expressed sequence tag-simple sequence repeat (EST-SSR) markers to investigate the sequence level genome duplication.
resultsReamplification and sequencing of the individual bands revealed that, for each marker, two bands around the expected size were genuine amplicons derived from two paralogous loci. In each case, one of the two bands was polymorphic, showing different allelic forms among nine ginseng cultivars, whereas the other band was usually monomorphic. Sequences derived from the two loci showed a high similarity, including the same primer-binding site, but each locus could be distinguished based on SSR number variations and additional single nucleotide polymorphisms (SNPs) or InDels. A locus-specific marker designed from the SNP site between the paralogous loci produced a single band that also showed clear polymorphism among ginseng cultivars.
conclusionOur data imply that the recent genome duplication has resulted in two highly similar paralogous regions in the ginseng genome. The two paralogous sequences could be differentiated by large SSR number variations and one or two additional SNPs or InDels in every 100 bp of genic region, which can serve as a reliable identifier for each locus.
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