ArticleNucleic acids research2014
The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation.Nature communications · 2026Article
- Spen and Nito prevent dedifferentiation of intermediate progenitors by maintaining Notch target expression in stem cells at low levels.Cell reports · 2026Article
- Article
- Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA.RNA (New York, N.Y.) · 2024Article
- IndiSPENsable for X Chromosome Inactivation and Gene Silencing.Epigenomes · 2023Review
- Repression of rRNA gene transcription by endothelial SPEN deficiency normalizes tumor vasculature via nucleolar stress.The Journal of clinical investigation · 2023Article
- Limited population genetic variation but pronounced seascape genetic structuring in populations of the Mediterranean mussel (Ecology and evolution · 2023Article
- Getting to the bottom of lncRNA mechanism: structure-function relationships.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Xist spatially amplifies SHARP/SPEN recruitment to balance chromosome-wide silencing and specificity to the X chromosome.Nature structural & molecular biology · 2022Article
- Review
- NCBP3: A Multifaceted Adaptive Regulator of Gene Expression.Trends in biochemical sciences · 2021Review
- Progress toward understanding chromosome silencing by Xist RNA.Genes & development · 2020Review
- Article
- Challenges and perspectives for structural biology of lncRNAs-the example of the Xist lncRNA A-repeats.Journal of molecular cell biology · 2019Article
- Long Non-coding RNA Structure and Function: Is There a Link?Frontiers in physiology · 2018Review
- Mechanistic insights in X-chromosome inactivation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei.PLoS pathogens · 2017Article
- Function by Structure: Spotlights on Xist Long Non-coding RNA.Frontiers in molecular biosciences · 2017Review
- hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA.Nucleic acids research · 2016Article
- COOLAIR Antisense RNAs Form Evolutionarily Conserved Elaborate Secondary Structures.Cell reports · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Split Ends (SPEN) protein was originally discovered in Drosophila in the late 1990s. Since then, homologous proteins have been identified in eukaryotic species ranging from plants to humans. Every family member contains three predicted RNA recognition motifs (RRMs) in the N-terminal region of the protein. We have determined the crystal structure of the region of the human SPEN homolog that contains these RRMs-the SMRT/HDAC1 Associated Repressor Protein (SHARP), at 2.0 Å resolution. SHARP is a co-regulator of the nuclear receptors. We demonstrate that two of the three RRMs, namely RRM3 and RRM4, interact via a highly conserved interface. Furthermore, we show that the RRM3-RRM4 block is the main platform mediating the stable association with the H12-H13 substructure found in the steroid receptor RNA activator (SRA), a long, non-coding RNA previously shown to play a crucial role in nuclear receptor transcriptional regulation. We determine that SHARP association with SRA relies on both single- and double-stranded RNA sequences. The crystal structure of the SHARP-RRM fragment, together with the associated RNA-binding studies, extend the repertoire of nucleic acid binding properties of RRM domains suggesting a new hypothesis for a better understanding of SPEN protein functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.