ArticleNucleic acids research2014
Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability.Toxins · 2018Pooled it
- Long Dynamic β1-β2 Loops inInternational journal of molecular sciences · 2024Article
- Structural and mutational analysis of MazE6-operator DNA complex provide insights into autoregulation of toxin-antitoxin systems.Communications biology · 2022Article
- Functional and Biochemical Characterization of the MazEF6 Toxin-Antitoxin System of Mycobacterium tuberculosis.Journal of bacteriology · 2022Article
- Role of PemI in the Staphylococcus aureus PemIK toxin-antitoxin complex: PemI controls PemK by acting as a PemK loop mimic.Nucleic acids research · 2022Article
- Combining cysteine scanning with chemical labeling to map protein-protein interactions and infer bound structure in an intrinsically disordered region.Frontiers in molecular biosciences · 2022Article
- Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA.Nucleic acids research · 2021Article
- Structural and functional analysis of theIUCrJ · 2021Article
- Functional and structural characterization of Deinococcus radiodurans R1 MazEF toxin-antitoxin system, Dr0416-Dr0417.Journal of microbiology (Seoul, Korea) · 2021Article
- MazEF Toxin-Antitoxin System-Mediated DNA Damage Stress Response inFrontiers in genetics · 2021Article
- mRNA InterferaseToxins · 2020Article
- The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.Frontiers in genetics · 2020Review
- YjbH Solubility Controls Spx inFrontiers in microbiology · 2020Article
- The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA.The Journal of biological chemistry · 2018Article
- Structural analyses of the MazEF4 toxin-antitoxin pair inThe Journal of biological chemistry · 2017Article
- The structure and function ofThe Journal of biological chemistry · 2017Article
- Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon.Nucleic acids research · 2017Article
- Structure, Biology, and Therapeutic Application of Toxin-Antitoxin Systems in Pathogenic Bacteria.Toxins · 2016Review
- Substrate Recognition and Activity Regulation of the Escherichia coli mRNA Endonuclease MazF.The Journal of biological chemistry · 2016Article
- Toxin-Antitoxin Systems of Staphylococcus aureus.Toxins · 2016Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE.
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