ReviewHistochemistry and cell biology2014
Progress in quantitative single-molecule localization microscopy.
Review in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Near-zero photon bioimaging by fusing deep learning and ultralow-light microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Imaging the endocannabinoid signaling system.Journal of neuroscience methods · 2022Review
- Quantitative live-cell PALM reveals nanoscopic Faa4 redistributions and dynamics on lipid droplets during metabolic transitions of yeast.Molecular biology of the cell · 2021Article
- A workflow for sizing oligomeric biomolecules based on cryo single molecule localization microscopy.PloS one · 2021Article
- Single-molecule localization microscopy.Nature reviews. Methods primers · 2021Article
- Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.Nature communications · 2020Article
- Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model.PLoS computational biology · 2020Article
- Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.Science advances · 2020Article
- Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors.Biophysical reviews · 2019Review
- Super-resolution Microscopy for Nanomedicine Research.ACS nano · 2019Article
- Histochemistry and Cell Biology: 61 years and not tired at all.Histochemistry and cell biology · 2019Article
- A tessellation-based colocalization analysis approach for single-molecule localization microscopy.Nature communications · 2019Article
- The Rényi divergence enables accurate and precise cluster analysis for localization microscopy.Bioinformatics (Oxford, England) · 2018Article
- MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions.The Journal of cell biology · 2018Article
- Spatial and temporal organization of cadherin in punctate adherens junctions.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Correlation analysis framework for localization-based superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the advent of single-molecule localization microscopy (SMLM) techniques, intracellular proteins can be imaged at unprecedented resolution with high specificity and contrast. These techniques can lead to a better understanding of cell functioning, as they allow, among other applications, counting the number of molecules of a protein specie in a single cell, studying the heterogeneity in protein spatial organization, and probing the spatial interactions between different protein species. However, the use of these techniques for accurate quantitative measurements requires corrections for multiple inherent sources of error, including: overcounting due to multiple localizations of a single fluorophore (i.e., photoblinking), undercounting caused by incomplete photoconversion, uncertainty in the localization of single molecules, sample drift during the long imaging time, and inaccurate image registration in the case of dual-color imaging. In this paper, we review recent efforts that address some of these sources of error in quantitative SMLM and give examples in the context of photoactivated localization microscopy (PALM).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.