ArticlePloS one2014
Acetobixan, an inhibitor of cellulose synthesis identified by microbial bioprospecting.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- A fungal natural product that inhibits plant cellulose biosynthesis by disrupting cellulose synthase complexes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A cellulose synthesis inhibitor affects cellulose synthase complex secretion and cortical microtubule dynamics.Plant physiology · 2024Article
- Breaking-Down Barriers: Proposal of Using Cellulose Biosynthesis Inhibitors and Cellulase Enzyme as a Novel Treatment Modality for Vision Threatening Pythium Insidiosum Keratitis.Clinical ophthalmology (Auckland, N.Z.) · 2024Article
- Genome Editing-Based Engineering of CESA3 Dual Cellulose-Inhibitor-Resistant Plants.Plant physiology · 2019Article
- Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens.Scientific reports · 2018Article
- Plant Chemical Genetics: From Phenotype-Based Screens to Synthetic Biology.Plant physiology · 2017Review
- Mitochondrial Defects Confer Tolerance against Cellulose Deficiency.The Plant cell · 2016Article
- The Emerging Role of Protein Phosphorylation as a Critical Regulatory Mechanism Controlling Cellulose Biosynthesis.Frontiers in plant science · 2016Review
- From the nucleus to the apoplast: building the plant’s cell wall.Journal of experimental botany · 2016Article
- 2-Fluoro-L-Fucose Is a Metabolically Incorporated Inhibitor of Plant Cell Wall Polysaccharide Fucosylation.PloS one · 2015Article
- Plant chemical biology: are we meeting the promise?Frontiers in plant science · 2014Article
- Experimental approaches to study plant cell walls during plant-microbe interactions.Frontiers in plant science · 2014Review
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Authors and funding
9 authors at 5 institutions in 1 country.
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Abstract
In plants, cellulose biosynthesis is an essential process for anisotropic growth and therefore is an ideal target for inhibition. Based on the documented utility of small-molecule inhibitors to dissect complex cellular processes we identified a cellulose biosynthesis inhibitor (CBI), named acetobixan, by bio-prospecting among compounds secreted by endophytic microorganisms. Acetobixan was identified using a drug-gene interaction screen to sift through hundreds of endophytic microbial secretions for one that caused synergistic reduction in root expansion of the leaky AtcesA6prc1-1 mutant. We then mined this microbial secretion for compounds that were differentially abundant compared with Bacilli that failed to mimic CBI action to isolate a lead pharmacophore. Analogs of this lead compound were screened for CBI activity, and the most potent analog was named acetobixan. In living Arabidopsis cells visualized by confocal microscopy, acetobixan treatment caused CESA particles localized at the plasma membrane (PM) to rapidly re-localize to cytoplasmic vesicles. Acetobixan inhibited 14C-Glc uptake into crystalline cellulose. Moreover, cortical microtubule dynamics were not disrupted by acetobixan, suggesting specific activity towards cellulose synthesis. Previous CBI resistant mutants such as ixr1-2, ixr2-1 or aegeus were not cross resistant to acetobixan indicating that acetobixan targets a different aspect of cellulose biosynthesis.
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