ArticleCellular and molecular life sciences : CMLS2014
Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.
Article in Cellular and molecular life sciences : CMLS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Minimization of Cancellation Effect with Nisin During Bipolar Nanosecond Electrochemotherapy.International journal of molecular sciences · 2026Article
- NaThe Journal of membrane biology · 2026Article
- Evaluation of unipolar and bipolar nanosecond pulses for calcium electrochemotherapy and immune response.Frontiers in immunology · 2026Article
- MHz compression of pulse packets facilitates remote focusing of electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2025Article
- The Effects of Bipolar Cancellation Phenomenon on Nano-Electrochemotherapy of Melanoma Tumors: In Vitro and In Vivo Pilot.International journal of molecular sciences · 2024Article
- Modulating CaBiophysical journal · 2024Article
- Threshold Interphase Delay for Bipolar Pulses to Prevent Cancellation Phenomenon during Electrochemotherapy.International journal of molecular sciences · 2024Article
- Integrated Time Nanosecond Pulse Irreversible Electroporation (INSPIRE): Assessment of Dose, Temperature, and Voltage on Experimental and Clinical Treatment Outcomes.IEEE transactions on bio-medical engineering · 2024Article
- Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses.Frontiers in bioengineering and biotechnology · 2024Article
- Ultra-Low Intensity Post-Pulse Affects Cellular Responses Caused by Nanosecond Pulsed Electric Fields.Bioengineering (Basel, Switzerland) · 2023Article
- Next generation CANCAN focusing for remote stimulation by nanosecond electric pulses.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction.International journal of molecular sciences · 2023Article
- Action spectra and mechanisms of (in) efficiency of bipolar electric pulses at electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Nanosecond PEF Induces Oxidative Stress and Apoptosis via Proteasomal Activity Inhibition in Gastric Adenocarcinoma Cells with Drug Resistance.International journal of molecular sciences · 2022Article
- Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation.Scientific reports · 2022Article
- Does the shape of the electric pulse matter in electroporation?Frontiers in oncology · 2022Review
- An Investigation for Large Volume, Focal Blood-Brain Barrier Disruption with High-Frequency Pulsed Electric Fields.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Electrotherapies for Glioblastoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Effects of high-frequency nanosecond pulses on prostate cancer cells.Scientific reports · 2021Article
- A Theoretical Argument for Extended Interpulse Delays in Therapeutic High-Frequency Irreversible Electroporation Treatments.IEEE transactions on bio-medical engineering · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Nanoelectroporation of biomembranes is an effect of high-voltage, nanosecond-duration electric pulses (nsEP). It occurs both in the plasma membrane and inside the cell, and nanoporated membranes are distinguished by ion-selective and potential-sensitive permeability. Here we report a novel phenomenon of bioeffects cancellation that puts nsEP cardinally apart from the conventional electroporation and electrostimulation by milli- and microsecond pulses. We compared the effects of 60- and 300-ns monopolar, nearly rectangular nsEP on intracellular Ca(2+) mobilization and cell survival with those of bipolar 60 + 60 and 300 + 300 ns pulses. For diverse endpoints, exposure conditions, pulse numbers (1-60), and amplitudes (15-60 kV/cm), the addition of the second phase cancelled the effects of the first phase. The overall effect of bipolar pulses was profoundly reduced, despite delivering twofold more energy. Cancellation also took place when two phases were separated into two independent nsEP of opposite polarities; it gradually tapered out as the interval between two nsEP increased, but was still present even at a 10-µs interval. The phenomenon of cancellation is unique for nsEP and has not been predicted by the equivalent circuit, transport lattice, and molecular dynamics models of electroporation. The existing paradigms of membrane permeabilization by nsEP will need to be modified. Here we discuss the possible involvement of the assisted membrane discharge, two-step oxidation of membrane phospholipids, and reverse transmembrane ion transport mechanisms. Cancellation impacts nsEP applications in cancer therapy, electrostimulation, and biotechnology, and provides new insights into effects of more complex waveforms, including pulsed electromagnetic emissions.
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