ArticlePloS one2014
Amoebal endosymbiont Neochlamydia genome sequence illuminates the bacterial role in the defense of the host amoebae against Legionella pneumophila.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
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- Retrospective Analysis of Drinking Water Microcosm Microbiomes Reveals an Apparent Antagonistic Relationship between and.Environmental science & technology letters · 2025Article
- Profile of Bacterial Communities in Copper Mine Tailings Revealed through High-Throughput Sequencing.Microorganisms · 2024Article
- Genomic analyses of Symbiomonas scintillans show no evidence for endosymbiotic bacteria but does reveal the presence of giant viruses.PLoS genetics · 2024Article
- Gene gain facilitated endosymbiotic evolution of Chlamydiae.Nature microbiology · 2023Article
- Complexities of Inferring Symbiont Function:Applied and environmental microbiology · 2022Article
- Novel Chlamydiae and Amoebophilus endosymbionts are prevalent in wild isolates of the model social amoeba Dictyostelium discoideum.Environmental microbiology reports · 2021Article
- Draft Genome Sequences ofMicrobiology resource announcements · 2020Article
- PeculiarFrontiers in microbiology · 2020Article
- Complete genome and bimodal genomic structure of the amoebal symbiont Neochlamydia strain S13 revealed by ultra-long reads obtained from MinION.Journal of human genetics · 2020Article
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- Lateral Gene Transfer Between Protozoa-Related Giant Viruses of FamilyEvolutionary bioinformatics online · 2018Article
- Environmental Metagenomic Assemblies Reveal Seven New Highly Divergent Chlamydial Lineages and Hallmarks of a Conserved Intracellular Lifestyle.Frontiers in microbiology · 2018Article
- From Many Hosts, One Accidental Pathogen: The Diverse Protozoan Hosts ofFrontiers in cellular and infection microbiology · 2017Review
- Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation.BMC microbiology · 2016Article
- CRISPR System Acquisition and Evolution of an Obligate Intracellular Chlamydia-Related Bacterium.Genome biology and evolution · 2016Article
- Draft Genome Sequences of Legionella pneumophila JR32 and Lp01 Laboratory Strains Domesticated in Japan.Genome announcements · 2016Article
- Ciliate Paramecium is a natural reservoir of Legionella pneumophila.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work has shown that the obligate intracellular amoebal endosymbiont Neochlamydia S13, an environmental chlamydia strain, has an amoebal infection rate of 100%, but does not cause amoebal lysis and lacks transferability to other host amoebae. The underlying mechanism for these observations remains unknown. In this study, we found that the host amoeba could completely evade Legionella infection. The draft genome sequence of Neochlamydia S13 revealed several defects in essential metabolic pathways, as well as unique molecules with leucine-rich repeats (LRRs) and ankyrin domains, responsible for protein-protein interaction. Neochlamydia S13 lacked an intact tricarboxylic acid cycle and had an incomplete respiratory chain. ADP/ATP translocases, ATP-binding cassette transporters, and secretion systems (types II and III) were well conserved, but no type IV secretion system was found. The number of outer membrane proteins (OmcB, PomS, 76-kDa protein, and OmpW) was limited. Interestingly, genes predicting unique proteins with LRRs (30 genes) or ankyrin domains (one gene) were identified. Furthermore, 33 transposases were found, possibly explaining the drastic genome modification. Taken together, the genomic features of Neochlamydia S13 explain the intimate interaction with the host amoeba to compensate for bacterial metabolic defects, and illuminate the role of the endosymbiont in the defense of the host amoebae against Legionella infection.
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