ArticleJournal of visualized experiments : JoVE2014
Examination of synaptic vesicle recycling using FM dyes during evoked, spontaneous, and miniature synaptic activities.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Differentiated SH-SY5Y cells exhibit neuronal features but lack synaptic maturity.Cell death discovery · 2026Article
- Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.Molecular neurobiology · 2025Article
- TRIB3 Links Endoplasmic Reticulum Stress to Impaired Efferocytosis in Atherosclerosis.Circulation research · 2025Article
- Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons.Scientific reports · 2025Article
- Stimulating VAPB-PTPIP51 ER-mitochondria tethering corrects FTD/ALS mutant TDP43 linked CaActa neuropathologica communications · 2024Article
- Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Spinogenesis and Synaptogenesis Effects of the Red SeaweedPreventive nutrition and food science · 2019Article
- The VAPB-PTPIP51 endoplasmic reticulum-mitochondria tethering proteins are present in neuronal synapses and regulate synaptic activity.Acta neuropathologica communications · 2019Article
- An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.Journal of visualized experiments : JoVE · 2018Article
- FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation.Journal of visualized experiments : JoVE · 2018Article
- ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA.Cell death & disease · 2018Article
- Teaching Dose-response Relationships Through Aminoglycoside Block of Mechanotransduction Channels in Lateral Line Hair Cells of Larval Zebrafish.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018Article
- Structure of the Golgi apparatus is not influenced by a GAG deletion mutation in the dystonia-associated gene Tor1a.PloS one · 2018Article
- IGF-II promotes neuroprotection and neuroplasticity recovery in a long-lasting model of oxidative damage induced by glucocorticoids.Redox biology · 2017Article
- Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons.Stem cells and development · 2016Article
- ABCA13 dysfunction associated with psychiatric disorders causes impaired cholesterol trafficking.The Journal of biological chemistryArticle
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic vesicles in functional nerve terminals undergo exocytosis and endocytosis. This synaptic vesicle recycling can be effectively analyzed using styryl FM dyes, which reveal membrane turnover. Conventional protocols for the use of FM dyes were designed for analyzing neurons following stimulated (evoked) synaptic activity. Recently, protocols have become available for analyzing the FM signals that accompany weaker synaptic activities, such as spontaneous or miniature synaptic events. Analysis of these small changes in FM signals requires that the imaging system is sufficiently sensitive to detect small changes in intensity, yet that artifactual changes of large amplitude are suppressed. Here we describe a protocol that can be applied to evoked, spontaneous, and miniature synaptic activities, and use cultured hippocampal neurons as an example. This protocol also incorporates a means of assessing the rate of photobleaching of FM dyes, as this is a significant source of artifacts when imaging small changes in intensity.
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