ArticleApplied and environmental microbiology2014
Variables influencing extraction of nucleic acids from microbial plankton (viruses, bacteria, and protists) collected on nanoporous aluminum oxide filters.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
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- Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.Nature communications · 2024Article
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- Distinct and rich assemblages of giant viruses in Arctic and Antarctic lakes.ISME communications · 2024Article
- Ubiquitous, BISME communications · 2023Article
- Arctic bacterial diversity and connectivity in the coastal margin of the Last Ice Area.ISME communications · 2023Article
- Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration.Food and environmental virology · 2023Article
- A Tale of Two Seasons: Distinct Seasonal Viral Communities in a Thermokarst Lake.Microorganisms · 2023Article
- Climate-Endangered Arctic Epishelf Lake Harbors Viral Assemblages with Distinct Genetic Repertoires.Applied and environmental microbiology · 2022Article
- Broad phylogenetic and functional diversity among mixotrophic consumers of Prochlorococcus.The ISME journal · 2022Article
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- Silicon limitation facilitates virus infection and mortality of marine diatoms.Nature microbiology · 2019Article
- Communities of Phytoplankton Viruses across the Transition Zone of the St. Lawrence Estuary.Viruses · 2018Article
- Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood.Journal of biomolecular techniques : JBT · 2018Article
- Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays.Science advances · 2016Article
- Molecular detection of airborne Coccidioides in Tucson, Arizona.Medical mycology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anodic aluminum oxide (AAO) filters have high porosity and can be manufactured with a pore size that is small enough to quantitatively capture viruses. These properties make the filters potentially useful for harvesting total microbial communities from water samples for molecular analyses, but their performance for nucleic acid extraction has not been systematically or quantitatively evaluated. In this study, we characterized the flux of water through commercially produced nanoporous (0.02 μm) AAO filters (Anotop; Whatman) and used isolates (a virus, a bacterium, and a protist) and natural seawater samples to test variables that we expected would influence the efficiency with which nucleic acids are recovered from the filters. Extraction chemistry had a significant effect on DNA yield, and back flushing the filters during extraction was found to improve yields of high-molecular-weight DNA. Using the back-flush protocol, the mass of DNA recovered from microorganisms collected on AAO filters was ≥ 100% of that extracted from pellets of cells and viruses and 94% ± 9% of that obtained by direct extraction of a liquid bacterial culture. The latter is a minimum estimate of the relative recovery of microbial DNA, since liquid cultures include dissolved nucleic acids that are retained inefficiently by the filter. In conclusion, we demonstrate that nucleic acids can be extracted from microorganisms on AAO filters with an efficiency similar to that achievable by direct extraction of microbes in suspension or in pellets. These filters are therefore a convenient means by which to harvest total microbial communities from multiple aqueous samples in parallel for subsequent molecular analyses.
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