ArticleNature nanotechnology2014
Quantitative imaging of single mRNA splice variants in living cells.
Article in Nature nanotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 164 citations in OpenAlex.
- Technologies to measure and modulate protein subcellular localization.Nature reviews. Molecular cell biology · 2026Review
- A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Innovative Applications of Nanopore Technology in Tumor Screening: An Exosome-Centric Approach.Biosensors · 2025Review
- Plasmonic biosensors and actuators for integrated point-of-care diagnostics.Npj biosensing · 2025Review
- Nanoplasmonic biosensors for environmental sustainability and human health.Chemical Society reviews · 2024Review
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- Dual/Multi-Modal Image-Guided Diagnosis and Therapy Based on Luminogens with Aggregation-Induced Emission.Molecules (Basel, Switzerland) · 2024Review
- DNA-Driven Dynamic Assembly/Disassembly of Inorganic Nanocrystals for Biomedical Imaging.Chemical & biomedical imaging · 2023Review
- Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter.Nature communications · 2023Article
- Isolation, Detection and Analysis of Circulating Tumour Cells: A Nanotechnological Bioscope.Pharmaceutics · 2023Review
- Multifunctional Oxygenated Particles for Targeted Cancer Drug Delivery and Evaluation with Darkfield Hyperspectral Imaging.ACS omega · 2022Article
- Dynamic observations of CRISPR-Cas target recognition and cleavage heterogeneities.Nanophotonics (Berlin, Germany) · 2022Article
- Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.Nucleic acids research · 2022Article
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Design of an Intron-Retained Bioluminescence Reporter and its Application in Imaging of Pre-mRNA Splicing in Living Subjects.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes.Nature communications · 2021Article
- Plasmonic gold nanostructures for biosensing and bioimaging.Mikrochimica acta · 2021Review
- The video-rate imaging of sub-10 nm plasmonic nanoparticles in a cellular medium free of background scattering.Chemical science · 2021Article
- Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging.Nature protocols · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Alternative messenger RNA (mRNA) splicing is a fundamental process of gene regulation, and errors in RNA splicing are known to be associated with a variety of different diseases. However, there is currently a lack of quantitative technologies for monitoring mRNA splice variants in cells. Here, we show that a combination of plasmonic dimer probes and hyperspectral imaging can be used to detect and quantify mRNA splice variants in living cells. The probes are made from gold nanoparticles functionalized with oligonucleotides and can hybridize to specific mRNA sequences, forming nanoparticle dimers that exhibit distinct spectral shifts due to plasmonic coupling. With this approach, we show that the spatial and temporal distribution of three selected splice variants of the breast cancer susceptibility gene, BRCA1, can be monitored at single-copy resolution by measuring the hybridization dynamics of the nanoplasmonic dimers. Our study provides insights into RNA and its transport in living cells, which could improve our understanding of cellular protein complexes, pharmacogenomics, genetic diagnosis and gene therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.