ArticleJournal of visualized experiments : JoVE2014
Mouse genome engineering using designer nucleases.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Mouse Models to Examine Differentiated Thyroid Cancer Pathogenesis: Recent Updates.International journal of molecular sciences · 2023Review
- Article
- LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.The EMBO journal · 2018Article
- Production of knock-in mice in a single generation from embryonic stem cells.Nature protocols · 2017Article
- Pramel7 mediates ground-state pluripotency through proteasomal-epigenetic combined pathways.Nature cell biology · 2017Article
- Genome-wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification.Molecular therapy. Methods & clinical development · 2017Article
- Long-Term Assessment of AAV-Mediated Zinc Finger Nuclease Expression in the Mouse Brain.Frontiers in molecular neuroscience · 2017Article
- Strictly co-isogenic C57BL/6J-Prnp-/- mice: A rigorous resource for prion science.The Journal of experimental medicine · 2016Article
- Mouse models of thyroid cancer: A 2015 update.Molecular and cellular endocrinology · 2016Review
- TALEN-mediated genome engineering to generate targeted mice.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Mouse Genome Editing Using the CRISPR/Cas System.Current protocols in human genetics · 2014Article
- The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.Transgenic research · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic mice carrying site-specific genome modifications (knockout, knock-in) are of vital importance for dissecting complex biological systems as well as for modeling human diseases and testing therapeutic strategies. Recent advances in the use of designer nucleases such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system for site-specific genome engineering open the possibility to perform rapid targeted genome modification in virtually any laboratory species without the need to rely on embryonic stem (ES) cell technology. A genome editing experiment typically starts with identification of designer nuclease target sites within a gene of interest followed by construction of custom DNA-binding domains to direct nuclease activity to the investigator-defined genomic locus. Designer nuclease plasmids are in vitro transcribed to generate mRNA for microinjection of fertilized mouse oocytes. Here, we provide a protocol for achieving targeted genome modification by direct injection of TALEN mRNA into fertilized mouse oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.