ArticleMolecular and cellular biology1989
E1a transactivation of the human HSP70 promoter is mediated through the basal transcriptional complex.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 144 citations in OpenAlex.
- HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells.The EMBO journal · 2014Article
- Down-regulation of heat shock protein HSP90ab1 in radiation-damaged lung cells other than mast cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Identification of a tissue-selective heat shock response regulatory network.PLoS genetics · 2013Article
- Basal and stress-induced Hsp70 are modulated by ataxin-3.Cell stress & chaperones · 2012Article
- Induction of heat shock proteins by hyperthermia and noise overstimulation in hsf1 -/- mice.Journal of the Association for Research in Otolaryngology : JARO · 2012Article
- Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions.Journal of molecular histology · 2008Article
- The induction levels of heat shock protein 70 differentiate the vulnerabilities to mutant huntingtin among neuronal subtypes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Cellular stress rather than stage of the cell cycle enhances the replication and plating efficiencies of herpes simplex virus type 1 ICP0- viruses.Journal of virology · 2006Article
- Arctigenin from Fructus Arctii is a novel suppressor of heat shock response in mammalian cells.Cell stress & chaperones · 2006Article
- Molecular chaperones and the stress of oncogenesis.Oncogene · 2004Article
- Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia.Cell stress & chaperones · 2001Article
- Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1.The EMBO journal · 2001Article
- The balance of nuclear import and export determines the intracellular distribution and function of tomato heat stress transcription factor HsfA2.Molecular and cellular biology · 2001Article
- HSP70 expression is increased during the day in a diurnal animal, the golden-mantled ground squirrel Spermophilus lateralis.Molecular and cellular biochemistry · 1999Article
- A cellular repressor of E1A-stimulated genes that inhibits activation by E2F.Molecular and cellular biology · 1998Article
- Expression levels of heat shock factors are not functionally coupled to the rate of expression of heat shock genes.Molecular biology reports · 1998Article
- Hsp70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- A novel G1-specific enhancer identified in the human heat shock protein 70 gene.Nucleic acids research · 1997Article
- Cloning and characterization of mouse CCAAT binding factor.Nucleic acids research · 1996Article
- Regulation of brain interleukin-1 beta (IL-1 beta) system mRNAs in response to pathophysiological concentrations of IL-1 beta in the cerebrospinal fluid.Journal of molecular neuroscience : MN · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the promoter sequence requirements for E1a transactivation of the human HSP70 gene by using a transient cotransfection assay. A 5' deletion study has defined a basal transcription unit extending to -74 relative to the transcription initiation site which was fully E1a responsive. Further deletion, abolishing a CCAAT element at -67, drastically reduced basal and E1a-induced expression. A linker-scanner analysis has identified four functional elements within the basal transcription unit which may interact with CTF, SP1, TFIID, and an ATF/AP1-like factor. Sequences between -100 and -188 can partially compensate for mutations in these elements. No mutation specifically abolished E1a inducibility. Any reduction in absolute E1a-induced levels was accompanied by a corresponding reduction in absolute basal levels, thereby maintaining a constant relative fold induction. We conclude that E1a transactivation of the human HSP70 promoter does not require any single basal transcription element. We also examined an HSP70 promoter fragment, containing the CCAAT element at -67 and the purine-rich element at -54, out of its normal context by fusing it upstream of a transcriptionally inactive herpes simplex virus thymidine kinase deletion construct containing only the TATA box. The resulting chimeric promoter was fully E1a responsive. Mutagenesis of this promoter fusion demonstrated that the CCAAT element was essential for detectable basal and E1a-induced expression. Mutations in the purine-rich element resulted in an approximately 10-fold elevation in basal levels and rendered the promoter nonresponsive to E1a.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.